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Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650) is a rat monoclonal antibody that is used to detect Myeloperoxidase in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (AB300650), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (AB300650), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (AB300650), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (AB300650), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (AB300650), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/10000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/10000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).

Alternative names

Myeloperoxidase, MPO

Recommended products

Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650) is a rat monoclonal antibody that is used to detect Myeloperoxidase in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20257
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Myeloperoxidase antibody [EPR20257] ab208670). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Associated diseases and disorders

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on human spleen.
    The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on human gastric carcinoma.
    The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on mouse spleen.
    The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
    Positive staining on rat spleen.
    The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling Myeloperoxidase with ab300650 at 1/50 dilution (20.26 µg/ml), followed by Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
    Confocal image showing cytoplasmic staining in HL-60 cell line.
    Negative control: HeLa (PMID: 12040446).
    Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
    -ve control 1: ab300650 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution.
    -ve control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at a 1/200 dilution followed by Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161 at a 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized mouse blood cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg)/ Right compared with a rat monoclonal IgG / Left. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) (ab300650)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
    Negative control: HeLa (PMID: 12040446).

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