AB300651

Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free

BOND RX™ Validated
Recombinant
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(1 Publication)

Rat Recombinant Monoclonal Myeloperoxidase antibody. Carrier free. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Myeloperoxidase, MPO

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300647, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300650, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 12040446).

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300650, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling Myeloperoxidase with ab300650 at 1/50 dilution (20.26 µg/ml), followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in HL-60 cell line. Negative control : HeLa (PMID : 12040446). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab300650 at a 1/50 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab150161 at a 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300647, the same antibody clone in a different buffer formulation Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human gastric carcinoma. The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300650, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab300650 at 1/10000 dilution (0.101 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat spleen. The section was incubated with ab300650 for 30 mins at room temperature, followed by Goat Anti-rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

This data was developed using ab300650, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized mouse blood cells labeling Myeloperoxidase with ab300650 at 1/1000 dilution (0.1µg)/ Right compared with a rat monoclonal IgG / Left. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

Unconjugated

Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric)

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

EPR20257

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab300651 is a carrier free version of ab300650.

This rat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab208670). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography DEAE-C

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1546422 PubMed40270954

2025

The nature of the post-translational modifications of the autoantigen LL37 influences the autoreactive T-helper cell phenotype in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Lande,Anna Mennella,Raffaella Palazzo,Rebecca Favaro,Paola Facheris,Flavia Mancini,Giuseppe Ocone,Elisabetta Botti,Mario Falchi,Immacolata Pietraforte,Curdin Conrad,Luca Bianchi,Antonio Costanzo,Loredana Frasca

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) - BSA and Azide free (AB300651)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise