AB134132

Anti-Myeloperoxidase antibody [EPR4793]

RabMAb
Recombinant
20ul selling size
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(12 Publications)

Rabbit Recombinant Monoclonal Myeloperoxidase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.

View Alternative Names

Myeloperoxidase, MPO

4 Images

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HL-60 (Human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with purified ab134132 at 1/10000 dilution (0.1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Negative control : HeLa.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Myeloperoxidase with Purified ab134132 at 1 : 1000 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Unknown

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

All lanes:

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution

All lanes:

HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 83 kDa

false

Unknown

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

All lanes:

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution

All lanes:

Human spleen lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 83 kDa

false

Carrier free

Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free
660 APC

APC Anti-Myeloperoxidase antibody [EPR4793]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myeloperoxidase antibody [EPR4793]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Myeloperoxidase antibody [EPR4793]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Myeloperoxidase antibody [EPR4793]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Myeloperoxidase antibody [EPR4793]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Myeloperoxidase antibody [EPR4793]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Myeloperoxidase antibody [EPR4793]
HRP

HRP Anti-Myeloperoxidase antibody [EPR4793]
578 PE

PE Anti-Myeloperoxidase antibody [EPR4793]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4793

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody recognizes Myeloperoxidase (85-90 kDa) and its heavy chain (60 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "For unpurified use at dilution between 1/250 to 1/500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 13:e70163 PubMed40018991

2025

MiR-146a Reduces Inflammation in Experimental Pancreatitis via the TRAF6-NF-κB Signaling Pathway in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Yuping Ren,Xueyang Li,Liang Xia,Jianhua Wan

Cancer biology & medicine 21: PubMed38172525

2024

Cancer-educated neutrophils promote lung cancer progression PARP-1-ALOX5-mediated MMP-9 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Han,Yuxin Chen,Nan Huang,Xiaowan Zhou,Yanfang Lv,Huizhong Li,Dafei Chai,Junnian Zheng,Gang Wang

Signal transduction and targeted therapy 8:279 PubMed37491321

2023

Interaction between neutrophil extracellular traps and cardiomyocytes contributes to atrial fibrillation progression.

Applications

Unspecified application

Species

Unspecified reactive species

Li He,Ruiqi Liu,Honghua Yue,Xiaoxin Zhang,Xiaohui Pan,Yutao Sun,Jun Shi,Guonian Zhu,Chaoyi Qin,Yingqiang Guo

Annals of translational medicine 10:823 PubMed36034983

2022

Bone marrow is the preferred source for isolation of rat neutrophils and the subsequent acquisition of neutrophil extracellular traps.

Applications

Unspecified application

Species

Unspecified reactive species

Li He,Xiaohui Pan,Xiaoxin Zhang,Guonian Zhu,Ruiqi Liu,Chaoyi Qin

Oncology letters 22:823 PubMed34691250

2021

New prognostic factors and scoring system for patients with acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Kuang,Yang Wang,Xianghong Cao,Chuanmei Peng,Hui Gao

Theranostics 11:3839-3852 PubMed33664865

2021

Lactate-induced M2 polarization of tumor-associated macrophages promotes the invasion of pituitary adenoma by secreting CCL17.

Applications

Unspecified application

Species

Unspecified reactive species

Anke Zhang,Yuanzhi Xu,Houshi Xu,Jie Ren,Tong Meng,Yunjia Ni,Qingwei Zhu,Wen-Bo Zhang,Yuan-Bo Pan,Jiali Jin,Yunke Bi,Zhe Bao Wu,Shaojian Lin,Meiqing Lou

Annals of translational medicine 8:441 PubMed32395485

2020

Preoperative leukocytosis is associated with increased tumor-infiltrating neutrophil extracellular traps and worse outcomes in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Hu Lv,Meilin Weng,Huihui Wang,Juan P Cata,Wankun Chen,Changhong Miao

Cell death & disease 10:303 PubMed30944299

2019

Inhibition of miR-155 reduces impaired autophagy and improves prognosis in an experimental pancreatitis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Wan,Xiaoyu Yang,Yuping Ren,Xueyang Li,Yin Zhu,Ashley N Haddock,Baoan Ji,Liang Xia,Nonghua Lu

Journal of hematology & oncology 10:162 PubMed28985760

2017

An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia.

Applications

IHC-P

Species

Mouse

Zhisheng Her,Kylie Su Mei Yong,Kathirvel Paramasivam,Wilson Wei Sheng Tan,Xue Ying Chan,Sue Yee Tan,Min Liu,Yong Fan,Yeh Ching Linn,Kam Man Hui,Uttam Surana,Qingfeng Chen

Molecular pharmacology 91:49-57 PubMed27974636

2016

Myeloperoxidase Enhances Etoposide and Mitoxantrone-Mediated DNA Damage: A Target for Myeloprotection in Cancer Chemotherapy.

Applications

Species

Unspecified reactive species

Mandeep Atwal,Emma L Lishman,Caroline A Austin,Ian G Cowell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase antibody [EPR4793]

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

Western blot - Anti-Myeloperoxidase antibody [EPR4793] (AB134132)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise