AB125301

Anti-Myf5 antibody

Name: Anti-Myf5 antibody. Rabbit polyclonal (ab125301) | Abcam
Brand: Abcam Limited
SKU: AB125301
Rating: 5 (2 reviews)

(2 Reviews)

(20 Publications)

Rabbit Polyclonal MYF5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications.

View Alternative Names

BHLHC2, MYF5, Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2

1 Images

Unknown

Western blot - Anti-Myf5 antibody (AB125301)

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125301 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Myf5 antibody (ab125301) at 1 µg/mL

Lane 1:

Skeletal Muscle (Human) Tissue Lysate - Fetal Tissue at 10 µg

Lane 2:

Mouse Embryo E10 Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa,46 kDa,52 kDa,98 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of our polyclonal antibody, ab125301 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab125078.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myf5 also known as Myogenic Factor 5 is a member of the myogenic regulatory factor (MRF) family. It plays a critical role in muscle differentiation. Myf5 has a molecular mass of approximately 29 kDa. It is expressed predominantly in skeletal muscle tissues. The protein is observed early in muscle development marking the beginning stages where muscle precursor cells commit to becoming muscle cells.

Biological function summary

Myf5 functions as a transcription factor that initiates muscle differentiation. It acts as part of a larger complex interacting with other MRFs such as MyoD and MYF6. These factors together regulate the expression of genes necessary for myoblasts to develop into mature muscle fibers. Myf5 directly binds to the promoter regions of target genes initiating transcription that supports muscle cell lineage progression.

Pathways

The myogenesis pathway is heavily influenced by Myf5 activity. It works in tandem with MyoD in the early specification of myoblasts and their proliferation. Secondary to this Myf5 forms a feedback loop with proteins like MEF2 and Pax7 which reinforces muscle identity. Myf5 expression helps maintain the balance between myoblast proliferation and differentiation ensuring effective muscle regeneration and repair.

Muscular dystrophy exemplifies a condition closely associated with Myf5. The protein's expression levels and functionality often correlate with the disease affecting muscle development and integrity. Satellite cells which express Myf5 become important in muscle repair and dysfunctional Myf5 can contribute to disease progression. Another disorder influenced by Myf5 is rhabdomyosarcoma where aberrant expression disrupts normal myogenic regulatory pathways alongside MyoD dysregulation potentially leading to tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed : 29887215). Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

See full target information MYF5

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 103:104075 PubMed39094501

2024

In ovo betaine injection improves breast muscle growth in newly hatched goslings through FXR/IGF-2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Jie Liu,Yulan Zhao,Yan Wang,Ruqian Zhao

Fish physiology and biochemistry 50:1527-1544 PubMed38733450

2024

Pikeperch muscle tissues: a comparative study of structure, enzymes, genes, and proteins in wild and farmed fish.

Applications

Unspecified application

Species

Unspecified reactive species

Katrin Tönißen,George P Franz,Elke Albrecht,Philipp Lutze,Ralf Bochert,Bianka Grunow

Medicina (Kaunas, Lithuania) 60: PubMed38541159

2024

Whey Peptide Alleviates Muscle Atrophy by Strongly Regulating Myocyte Differentiation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jin A Lee,Mi-Rae Shin,Minju Kim,Hwa-Young Kim,Hwang-Yong Choi,Yoojin Seo,Hakjoo Choi,Seong-Soo Roh

Journal of animal science 101: PubMed37688555

2023

Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress.

Applications

Unspecified application

Species

Unspecified reactive species

Won Seob Kim,Jayasimha R Daddam,Boon Hong Keng,Jaehwan Kim,Jongkyoo Kim

Intractable & rare diseases research 12:180-190 PubMed37662621

2023

The novel role of IFITM1-3 in myogenic differentiation of C2C12 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtao Zhang,Yanqin Lu,Xianxian Li,Shanshan Zhang,Pengchao Liu,Xiaoyang Hao,Jinxiang Han

Journal of nanobiotechnology 21:304 PubMed37644475

2023

Human milk extracellular vesicles enhance muscle growth and physical performance of immature mice associating with Akt/mTOR/p70s6k signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zitong Meng,Dong Zhou,Dan Lv,Quan Gan,Yuxiao Liao,Zhao Peng,Xiaolei Zhou,Shiyin Xu,Penglong Chi,Zhipeng Wang,Andreas K Nüssler,Xuefeng Yang,Liegang Liu,Dongrui Deng,Wei Yang

Cells 12: PubMed37681878

2023

GABA Prevents Age-Related Sarcopenic Obesity in Mice with High-Fat-Diet-Induced Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Heegu Jin,Hyun-Ji Oh,Boo-Yong Lee

The Journal of physiology 601:961-978 PubMed36715084

2023

Lipin1 plays complementary roles in myofibre stability and regeneration in dystrophic muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahman Jama,Abdullah A Alshudukhi,Steve Burke,Lixin Dong,John Karanja Kamau,Andrew Alvin Voss,Hongmei Ren

NPJ Regenerative medicine 7:63 PubMed36266362

2022

Myogenic tissue nanotransfection improves muscle torque recovery following volumetric muscle loss.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Clark,Subhadip Ghatak,Poornachander Reddy Guda,Mohamed S El Masry,Yi Xuan,Amy Y Sato,Teresita Bellido,Chandan K Sen

Journal of cachexia, sarcopenia and muscle 12:1741-1756 PubMed34435457

2021

Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα mesenchymal progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Kai Shang,Lu Han,Zhi-Hao Wang,Ming Song,Di Wang,Yan-Min Tan,Yi-Hui Li,Yu-Lin Li,Wei Zhang,Ming Zhong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myf5 antibody

Western blot - Anti-Myf5 antibody (AB125301)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise