AB125078

Anti-Myf5 antibody [EPR4899]

20ul selling size
RabMAb
Recombinant
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(19 Publications)

Rabbit Recombinant Monoclonal MYF5 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

View Alternative Names

BHLHC2, MYF5, Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2

4 Images

Lab

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (AB125078)

ab125078 (Purified) at 1/20 dilution (20 μg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.

Lane 1 (input) : Human skeletal muscle
Lane 2 (+) : 125078 & Human skeletal muscle lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, ab125078 at 1/1000 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (ab125078)

Predicted band size: 28 kDa

false

Lab

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 39 kDa

false

Lab

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Myf5 antibody [EPR4899] (ab125078) at 1/50000 dilution

Lane 1:

Rat muscle tissue lysate at 10 µg

Lane 2:

Mouse muscle tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 39 kDa

false

Unknown

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

All lanes:

Western blot - Anti-Myf5 antibody [EPR4899] (ab125078) at 1/10000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Lane 3:

Mouse muscle tissue lysate at 10 µg

Lane 4:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 28 kDa

false

Carrier free

Anti-Myf5 antibody [EPR4899] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4899

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myf5 also known as Myogenic Factor 5 is a member of the myogenic regulatory factor (MRF) family. It plays a critical role in muscle differentiation. Myf5 has a molecular mass of approximately 29 kDa. It is expressed predominantly in skeletal muscle tissues. The protein is observed early in muscle development marking the beginning stages where muscle precursor cells commit to becoming muscle cells.

Biological function summary

Myf5 functions as a transcription factor that initiates muscle differentiation. It acts as part of a larger complex interacting with other MRFs such as MyoD and MYF6. These factors together regulate the expression of genes necessary for myoblasts to develop into mature muscle fibers. Myf5 directly binds to the promoter regions of target genes initiating transcription that supports muscle cell lineage progression.

Pathways

The myogenesis pathway is heavily influenced by Myf5 activity. It works in tandem with MyoD in the early specification of myoblasts and their proliferation. Secondary to this Myf5 forms a feedback loop with proteins like MEF2 and Pax7 which reinforces muscle identity. Myf5 expression helps maintain the balance between myoblast proliferation and differentiation ensuring effective muscle regeneration and repair.

Muscular dystrophy exemplifies a condition closely associated with Myf5. The protein's expression levels and functionality often correlate with the disease affecting muscle development and integrity. Satellite cells which express Myf5 become important in muscle repair and dysfunctional Myf5 can contribute to disease progression. Another disorder influenced by Myf5 is rhabdomyosarcoma where aberrant expression disrupts normal myogenic regulatory pathways alongside MyoD dysregulation potentially leading to tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed : 29887215). Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

See full target information MYF5

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40004006

2025

Human iPSC-Derived Muscle Cells as a New Model for Investigation of EDMD1 Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Lisowska,Marta Rowińska,Aleksandra Suszyńska,Claudia Bearzi,Izabela Łaczmańska,Julia Hanusek,Amanda Kunik,Volha Dzianisava,Ryszard Rzepecki,Magdalena Machowska,Katarzyna Piekarowicz

Stem cells international 2024:2812390 PubMed39758702

2025

Noggin Combined With Human Dental Pulp Stem Cells to Promote Skeletal Muscle Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Han Zhang,Li-Ming Yu,Wei-Hua Zhang,Jia-Jia Deng,Bing-Jing Sun,Mei-Hua Chen,Wei Huang,Jiao Li,Hua He,Xin-Xin Han,Yue-Hua Liu

BMC genomics 25:1146 PubMed39604828

2024

Lnc-MEG8 regulates yak myoblast differentiation via the miR-22-3p/RTL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Ma,Yongfu La,Tong Wang,Chun Huang,Fen Feng,Xian Guo,Pengjia Bao,Xiaoyun Wu,Min Chu,Chunnian Liang,Ping Yan

Cell death & disease 15:200 PubMed38459002

2024

p66Shc signaling and autophagy impact on C2C12 myoblast differentiation during senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yaiza Potes,Juan C Bermejo-Millo,Catarina Mendes,José P Castelão-Baptista,Andrea Díaz-Luis,Zulema Pérez-Martínez,Juan J Solano,Vilma A Sardão,Paulo J Oliveira,Beatriz Caballero,Ana Coto-Montes,Ignacio Vega-Naredo

Aging 15:13980-13997 PubMed38100482

2023

lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Yu,Xiuting He,Ting Liu,Jie Li

Physiological reports 11:e15756 PubMed37332022

2023

Exploring skeletal muscle tolerance and whole-body metabolic effects of FDA-approved drugs in a volumetric muscle loss model.

Applications

Unspecified application

Species

Unspecified reactive species

Shefali R Bijwadia,Christiana J Raymond-Pope,Alec M Basten,Mason T Lentz,Thomas J Lillquist,Jarrod A Call,Sarah M Greising

PloS one 18:e0281718 PubMed36763621

2023

Metformin regulates myoblast differentiation through an AMPK-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Maniscalco,Giuliana Abbadessa,Magalì Giordano,Loredana Grasso,Paolo Borrione,Silvia Racca

The Journal of biological chemistry 297:101101 PubMed34419449

2021

Retinoic acid exerts sexually dimorphic effects on muscle energy metabolism and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxin Zhao,Marta Vuckovic,Hong Sik Yoo,Nina Fox,Adrienne Rodriguez,Kyler McKessy,Joseph L Napoli

Aging 13:15044-15060 PubMed34051073

2021

Integrated analysis of long non-coding RNAs (lncRNAs) and mRNA expression profiles identifies lncRNA PRKG1-AS1 playing important roles in skeletal muscle aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zheng,Ting Liu,Qun Li,Jie Li

Molecular therapy. Nucleic acids 23:872-886 PubMed33614236

2021

Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Zezhong Mou,Zheyu Zhang,Siqi Wu,Quan Zhou,Yiling Chen,Jian Gong,Chenyang Xu,Yuxi Ou,Xinan Chen,Xiyu Dai,Haowen Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myf5 antibody [EPR4899]

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (AB125078)

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

Western blot - Anti-Myf5 antibody [EPR4899] (AB125078)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise