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AB240019

Anti-Myf5 antibody [EPR4899] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MYF5 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BHLHC2, MYF5, Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2

1 Images
Immunoprecipitation - Anti-Myf5 antibody [EPR4899] - BSA and Azide free (AB240019)
  • IP

Lab

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] - BSA and Azide free (AB240019)

ab125078 (Purified) at 1 : 1000 dilution (0.666 μg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input) : Human skeletal muscle
Lane 2 (+) : 125078 & Human skeletal muscle lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125078).

All lanes:

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (<a href='/en-us/products/primary-antibodies/myf5-antibody-epr4899-ab125078'>ab125078</a>)

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4899

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab240019 is the carrier-free version of ab125078.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myf5 also known as Myogenic Factor 5 is a member of the myogenic regulatory factor (MRF) family. It plays a critical role in muscle differentiation. Myf5 has a molecular mass of approximately 29 kDa. It is expressed predominantly in skeletal muscle tissues. The protein is observed early in muscle development marking the beginning stages where muscle precursor cells commit to becoming muscle cells.
Biological function summary

Myf5 functions as a transcription factor that initiates muscle differentiation. It acts as part of a larger complex interacting with other MRFs such as MyoD and MYF6. These factors together regulate the expression of genes necessary for myoblasts to develop into mature muscle fibers. Myf5 directly binds to the promoter regions of target genes initiating transcription that supports muscle cell lineage progression.

Pathways

The myogenesis pathway is heavily influenced by Myf5 activity. It works in tandem with MyoD in the early specification of myoblasts and their proliferation. Secondary to this Myf5 forms a feedback loop with proteins like MEF2 and Pax7 which reinforces muscle identity. Myf5 expression helps maintain the balance between myoblast proliferation and differentiation ensuring effective muscle regeneration and repair.

Muscular dystrophy exemplifies a condition closely associated with Myf5. The protein's expression levels and functionality often correlate with the disease affecting muscle development and integrity. Satellite cells which express Myf5 become important in muscle repair and dysfunctional Myf5 can contribute to disease progression. Another disorder influenced by Myf5 is rhabdomyosarcoma where aberrant expression disrupts normal myogenic regulatory pathways alongside MyoD dysregulation potentially leading to tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed : 29887215). Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
See full target information MYF5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 19:2575-2577 PubMed37194363

2023

Knockout validation of LAMP2A antibodies for immunostaining in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhou,Vera Shirokova,Vitaliy O Kaminskyy,Eva Berenger,Elena Kochetkova,Erik Norberg,Maria Genander,Helin Vakifahmetoglu-Norberg
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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