AB228722

Anti-MYH/MUTYH antibody

Name: Anti-MYH/MUTYH antibody (ab228722) | Abcam
Brand: Abcam Limited
SKU: AB228722
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal MYH/MUTYH antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MUTYH.

View Alternative Names

MYH, MUTYH, Adenine DNA glycosylase, MutY homolog, hMYH

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MYH/MUTYH antibody (AB228722)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYH/MUTYH antibody (AB228722)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MYH/MUTYH (green) using ab228722 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-MYH/MUTYH antibody (AB228722)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MYH/MUTYH antibody (ab228722) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-MYH/MUTYH antibody (AB228722)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MYH/MUTYH antibody (ab228722) at 1/1000 dilution

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MUTYH. The exact immunogen used to generate this antibody is proprietary information.

Q9UIF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/5000", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.

See full target information MUTYH

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 9:125 PubMed38114467

2023

Comprehensive genomic profiling of breast cancers characterizes germline-somatic mutation interactions mediating therapeutic vulnerabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Chen,Cai-Jin Lin,Yu-Chen Pei,Ding Ma,Li Liao,Si-Yuan Li,Lei Fan,Gen-Hong Di,Song-Yang Wu,Xi-Yu Liu,Yun-Jin Wang,Qi Hong,Guo-Liang Zhang,Lin-Lin Xu,Bei-Bei Li,Wei Huang,Jin-Xiu Shi,Yi-Zhou Jiang,Xin Hu,Zhi-Ming Shao

Frontiers in pharmacology 14:1220620 PubMed37576821

2023

PPAR-gamma agonist pioglitazone recovers mitochondrial quality control in fibroblasts from -deficient patients.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Di Donfrancesco,Christian Berlingieri,Marta Giacomello,Chiara Frascarelli,Ana Paula Magalhaes Rebelo,Laurence A Bindoff,Segel Reeval,Paul Renbaum,Filippo M Santorelli,Giulia Massaro,Carlo Viscomi,Massimo Zeviani,Daniele Ghezzi,Emanuela Bottani,Dario Brunetti

Scientific reports 12:19936 PubMed36402786

2022

Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lia Tesfay,Bibbin T Paul,Poornima Hegde,Molly Brewer,Samrin Habbani,Evan Jellison,Timothy Moore,Hao Wu,Suzy V Torti,Frank M Torti

Oxidative medicine and cellular longevity 2021:8635088 PubMed34970419

2021

MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Mizuno,Nona Abolhassani,Guianfranco Mazzei,Kunihiko Sakumi,Takashi Saito,Takaomi C Saido,Toshiharu Ninomiya,Toru Iwaki,Ryo Yamasaki,Jun-Ichi Kira,Yusaku Nakabeppu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYH/MUTYH antibody

Immunocytochemistry/ Immunofluorescence - Anti-MYH/MUTYH antibody (AB228722)

Western blot - Anti-MYH/MUTYH antibody (AB228722)

Western blot - Anti-MYH/MUTYH antibody (AB228722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise