AB232897

Anti-Myh14 antibody

Name: Anti-Myh14 antibody (ab232897) | Abcam
Brand: Abcam Limited
SKU: AB232897
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal MYH14 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MYH14 aa 1050-1350.

View Alternative Names

KIAA2034, FP17425, MYH14, Myosin-14, Myosin heavy chain 14, Non-muscle myosin heavy chain IIc, NMHC II-C

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Myh14 using ab232897 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Myh14 using ab232897 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Formalin-fixed, paraffin-embedded human liver tissue stained for Myh14 using ab232897 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Myh14 antibody (AB232897)

All lanes:

Western blot - Anti-Myh14 antibody (ab232897) at 1 µg/mL

All lanes:

Rat brain tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 228 kDa

false

Supplier Data

Western blot - Anti-Myh14 antibody (AB232897)

All lanes:

Western blot - Anti-Myh14 antibody (ab232897) at 1 µg/mL

All lanes:

Recombinant human Myh14 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 228 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MYH14 aa 1050-1350. The exact immunogen used to generate this antibody is proprietary information.

Q7Z406

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Myh14 also known as myosin-14 and non-muscle myosin heavy chain IIC is a member of the myosin superfamily of motor proteins. This protein is approximately 227 kDa in mass and plays an important role in cellular motility by facilitating actin-based transport processes. Myh14 is expressed in various tissues including the heart spleen and cerebellum suggesting its involvement in diverse physiological processes.

Biological function summary

Myh14 functions in the cytoskeleton interacting with actin filaments to contribute to processes like cell shape changes and migration. It acts as a part of the non-muscle myosin IIC complex working in tandem with other cytoskeletal elements to provide structural integrity and tension within cells. This activity supports various cellular mechanisms that require precise mechanical movement and stability.

Pathways

Myh14 plays a role in the Rho GTPase signaling pathway and actin cytoskeleton regulation. These pathways are important for cell adhesion and motility influencing how cells interact and communicate in their environments. Myh14's actions are closely related to proteins such as actin and other myosin family members which collectively maintain cytoskeletal dynamics and cellular responses to external signals.

Myh14 has been linked to sensorineural hearing loss and ischemic stroke. Its relationship with disorders involving the auditory system connects it to proteins like Myh9 another myosin family member associated with similar conditions. In cardiovascular pathology alterations in Myh14 expression may affect cellular responses during tissue damage such as those observed in stroke linking it to physiological processes and potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.

See full target information MYH14

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 14: PubMed38673008

2024

Alteration of the Expression and Functional Activities of Myosin II Isoforms in Enlarged Hyperplastic Prostates.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Weixiang He,Hui Chen,Rui Yang,Hongmei Su,Michael E DiSanto,Xinhua Zhang

Aging 13:23739-23756 PubMed34689137

2021

Loganin substantially ameliorates molecular deficits, pathologies and cognitive impairment in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lulin Nie,Kaiwu He,Fengzhu Xie,Shifeng Xiao,Shupeng Li,Jia Xu,Kaiqin Zhang,Chen Yang,Li Zhou,Jianjun Liu,Liangyu Zou,Xifei Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myh14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myh14 antibody (AB232897)

Western blot - Anti-Myh14 antibody (AB232897)

Western blot - Anti-Myh14 antibody (AB232897)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise