Anti-MYH2 antibody [EPR5280]

• WB

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Western blot - Anti-MYH2 antibody [EPR5280] (AB124937)

All lanes:

Western blot - Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution

Lane 1:

Mouse muscle tissue lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 223 kDa

Observed band size: 220 kDa

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• WB

Unknown

Western blot - Anti-MYH2 antibody [EPR5280] (AB124937)

All lanes:

Western blot - Anti-MYH2 antibody [EPR5280] (ab124937) at 1/10000 dilution

All lanes:

Human trachea lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 223 kDa

Observed band size: 220 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-MYH2 antibody [EPR5280] (AB124937)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-MYH2 antibody [EPR5280] (AB124937)

MYH2 western blot using anti-MYH2 antibody [EPR5280] ab124937. Publication image and figure legend from Zeng, X., Chen, S., et al., 2017, Oncotarget, PubMed 29069832.

ab124937 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124937 please see the product overview.

AG/UnAG prevents co-cultured myotubes breakdown(A) Immunofluorescence staining for anti-myosin heavy chain (MHC) antibody in C2C12 myotubes (× 100). MHC staining outlines the myotubes (green). 4'6-Diamidino-2-phenylindole was used to stain nuclei (blue). (B) Myotubes diameter expressed as % of negative control. Significant differences were detected between CO and any NC groups (#p < 0.001), between CO and CO+AG/UnAG groups (*p < 0.001), by one-way ANOVA followed by Tukey test. (C) The levels of MHC mRNA in C2C12 myotubes. mRNA levels were normalized to GAPDH and expressed as fold change from nagative control. Significant differences were detected between NC and CO groups (#p < 0.001), between CO and CO+AG/UnAG groups (*P = 0.044, P = 0.001; respectively), by one-way ANOVA followed by Dunnett's T3 test. (D) Western blot of MHC2, MHC7 and GAPDH in C2C12 myotubes. (E) Quantification of MHC2 was normalized to GAPDH. Significant differences were detected between CO and any NC groups (#P = 0.001, p < 0.001; P = 0.001 respectively), between CO and CO+AG/UnAG groups (*p < 0.001, P = 0.015; respectively), by one-way ANOVA followed by Tukey test. (F) Quantification of MHC7 was normalized to GAPDH. Significant differences were detected between CO and any NC groups (#P = 0.004, P = 0.001; P = 0.047 respectively), between CO and CO+AG/UnAG groups (*P = 0.035, P = 0.026; respectively), by one-way ANOVA followed by Tukey test. Data are represented as mean ± SD. Scale bar represents 100 μm.

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