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Rabbit Recombinant Monoclonal MYH6 antibody. Suitable for mIHC, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCWBIHC-PICC/IFFlow CytIHC-Fr
Human
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Target data

Function

Muscle contraction.

Alternative names

Recommended products

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Rabbit Recombinant Monoclonal MYH6 antibody. Suitable for mIHC, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10891(2)

Purification technique

Affinity purification Protein A

Specificity

The immunogen used for this product shares 78%,76%,76%,76%,72%homology with Myosin 2 1 3 8 4, respectively. Cross-reactivity with these proteins has not been confirmed experimentally.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967), expandable thumbnail

    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    ab185967 could recognize both Myosin 6 and 7 protein

    All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (AB185967) at 1/1000 dilution

    All lanes: His tagged recombinant Human Myosin-6 protein(aa 1193-1337) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967), expandable thumbnail

    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    ab185967 could recognize both Myosin 6 and 7 protein

    All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (AB185967) at 1/1000 dilution

    All lanes: His tagged recombinant Human Myosin-7 protein(aa 1191-1335) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

  • Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967), expandable thumbnail

    Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (AB185967) at 1/500 dilution

    Lane 1: Human adult heart lysate at 20 µg

    Lane 2: Mouse heart lysate at 20 µg

    Lane 3: Rat heart lysate at 20 µg

    Secondary

    All lanes: Goat anti Rabbit IR680 at 1/10000 dilution

    Predicted band size: 36 kDa, 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Multiplex immunohistochemistry - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967), expandable thumbnail

    Multiplex immunohistochemistry - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

    Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-delta Sarcoglycan (ab137101, red; Opal™690), anti-MYH2 (ab264036, cyan; Opal™520) and anti-MYH6 + Slow Skeletal Myosin Heavy chain (ab250868, gray; Opal™570) on human skeletal muscle. Panel B: anti-MYH2 stained on the fast twitch type 2A fibers. Panel C: anti-MYH6 + Slow Skeletal Myosin Heavy chain stained on skeletal muscle fiber subtypes. Panel D: anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 dilution (1.043 μg/ml), ab264036 at 1/1000 dilution (1.116 μg/ml) and ab250868 1/5000 dilution (0.209 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation (ab250868).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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