Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)]

5 product images

• 

  Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

  ab185967 could recognize both Myosin 6 and 7 protein

  All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution

  All lanes: His tagged recombinant Human Myosin-6 protein(aa 1193-1337) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

• 

  Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

  ab185967 could recognize both Myosin 6 and 7 protein

  All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/1000 dilution

  All lanes: His tagged recombinant Human Myosin-7 protein(aa 1191-1335) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

• 

  Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  All lanes: Western blot - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967) at 1/500 dilution

  Lane 1: Human adult heart lysate at 20 µg

  Lane 2: Mouse heart lysate at 20 µg

  Lane 3: Rat heart lysate at 20 µg

  Secondary

  All lanes: Goat anti Rabbit IR680 at 1/10000 dilution

  Predicted band size: 36 kDa, 41 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

  Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin.
  

  Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• 

  Multiplex immunohistochemistry - Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] (ab185967)

  Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-delta Sarcoglycan (Anti-delta Sarcoglycan antibody [EPR8706] ab137101, red; Opal™690), anti-MYH2 (Anti-MYH2 antibody [A4.74] ab264036, cyan; Opal™520) and anti-MYH6 + Slow Skeletal Myosin Heavy chain (Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free ab250868, gray; Opal™570) on human skeletal muscle. Panel B: anti-MYH2 stained on the fast twitch type 2A fibers. Panel C: anti-MYH6 + Slow Skeletal Myosin Heavy chain stained on skeletal muscle fiber subtypes. Panel D: anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-delta Sarcoglycan antibody [EPR8706] ab137101 at 1/1000 dilution (1.043 μg/ml), Anti-MYH2 antibody [A4.74] ab264036 at 1/1000 dilution (1.116 μg/ml) and Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free ab250868 1/5000 dilution (0.209 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
  
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  
  This data was developed using the same antibody clone in a different buffer formulation (Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free ab250868).