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AB172967

Anti-MYH7B antibody [EPR12290]

4

(3 Reviews)

|

(22 Publications)

Rabbit Recombinant Monoclonal MYH7B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 22 publications.

View Alternative Names

KIAA1512, MYH7B, Myosin-7B, Antigen MLAA-21, Myosin cardiac muscle beta chain, Slow A MYH14

10 Images
Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Supplier Data

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution

Lane 1:

Human fetal muscle lysates at 10 µg

Lane 2:

Human fetal heart lysates at 10 µg

Lane 3:

Human skeletal muscle lysates at 10 µg

Predicted band size: 221 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1 : 1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing -ve staining in Human normal breast tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing +ve staining in Human normal pancreas tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1 : 1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing -ve staining in Human normal liver tissue.

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Unknown

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/mL

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Rat heart lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa

Observed band size: 221 kDa

false

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Unknown

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/mL

All lanes:

Human fetal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa

Observed band size: 221 kDa

false

  • Carrier free

    Anti-MYH7B antibody [EPR12290] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12290

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified format use at 1/250 - 1/500. </strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in muscle contraction.
See full target information MYH7B
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 8:414 PubMed37857609

2023

Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wan,Zhen Zhang,Chennan Wu,Saisai Tian,Yibei Zang,Ge Jin,Qingyan Sun,Pin Wang,Xin Luan,Yili Yang,Xuelin Zhan,Lingyu Linda Ye,Dayue Darrel Duan,Xia Liu,Weidong Zhang

iScience 26:107826 PubMed37752946

2023

Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo M Lavecchia,Polyxeni Mantzouratou,Domenico Cerullo,Monica Locatelli,Sara Conti,Matteo Tironi,Fabio Sangalli,Daniela Corna,Carlamaria Zoja,Giuseppe Remuzzi,Christodoulos Xinaris

International journal of molecular sciences 24: PubMed37569807

2023

Inhibition of miR-25 Ameliorates Cardiac Dysfunction and Fibrosis by Restoring Krüppel-like Factor 4 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cholong Lee,Sunghye Cho,Dongtak Jeong

Journal of the Endocrine Society 6:bvac132 PubMed36249410

2022

Castration of Male Mice Induces Metabolic Remodeling of the Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Elin Svedlund Eriksson,Inger Johansson,Anna K F Mårtensson,Marta Lantero Rodriguez,Maaike Schilperoort,Jan Kroon,Sander Kooijman,Elmir Omerovic,Linda Andersson,Malin C Levin,Patrick C N Rensen,Åsa Tivesten

British journal of pharmacology 179:4575-4592 PubMed35751875

2022

Riociguat attenuates the changes in left ventricular proteome and microRNA profile after experimental aortic stenosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Benkner,Julia Rüdebusch,Neetika Nath,Elke Hammer,Karina Grube,Stefan Gross,Vishnu M Dhople,Gertrud Eckstein,Thomas Meitinger,Lars Kaderali,Uwe Völker,Jens Fielitz,Stephan B Felix

European journal of histochemistry : EJH 66: PubMed35726535

2022

MALAT1 regulates hypertrophy of cardiomyocytes by modulating the miR-181a/HMGB2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Chen,Wenfeng Li,Dandan Zhang,Youlin Fu,Wenjin Yuan,Gang Luo,Fuwei Liu,Jun Luo

Cell death discovery 8:167 PubMed35383152

2022

The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through mA RNA methylation reading protein Ythdf2.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqing Yang,Muisha B Mbikyo,Junzhe Zhang,Yuan Zhang,Naijin Zhang,Zhao Li

Diabetologia 65:563-581 PubMed34913989

2021

MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Yang,Guangyan Yang,Han Wu,Lin Kang,Jiaqing Xiang,Peilin Zheng,Shanhu Qiu,Zhen Liang,Yan Lu,Lijing Jia

Frontiers in pharmacology 12:766024 PubMed34925023

2021

Oxytocin Protects Against Isoproterenol-Induced Cardiac Hypertrophy by Inhibiting PI3K/AKT Pathway via a lncRNA GAS5/miR-375-3p/KLF4-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqiao Yang,Zhuoran Wang,Mengran Yao,Wei Xiong,Jun Wang,Yu Fang,Wei Yang,Haixia Jiang,Ning Song,Lan Liu,Jinqiao Qian

Oxidative medicine and cellular longevity 2021:6699054 PubMed33824698

2021

Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene.

Applications

Unspecified application

Species

Unspecified reactive species

Shengqi Huo,Wei Shi,Haiyan Ma,Dan Yan,Pengcheng Luo,Junyi Guo,Chenglong Li,Jiayuh Lin,Cuntai Zhang,Sheng Li,Jiagao Lv,Li Lin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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