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AB172967

Anti-MYH7B antibody [EPR12290]

4

(3 Reviews)

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(29 Publications)

Rabbit Recombinant Monoclonal MYH7B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 29 publications.

View Alternative Names

KIAA1512, MYH7B, Myosin-7B, Antigen MLAA-21, Myosin cardiac muscle beta chain, Slow A MYH14

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1 : 1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing -ve staining in Human normal breast tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing +ve staining in Human normal pancreas tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1 : 1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

ab172967 showing -ve staining in Human normal liver tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Supplier Data

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution

Lane 1:

Human fetal muscle lysates at 10 µg

Lane 2:

Human fetal heart lysates at 10 µg

Lane 3:

Human skeletal muscle lysates at 10 µg

Predicted band size: 221 kDa

false

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Unknown

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/mL

All lanes:

Human fetal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa

Observed band size: 221 kDa

false

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)
  • WB

Unknown

Western blot - Anti-MYH7B antibody [EPR12290] (AB172967)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/mL

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Rat heart lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 221 kDa

Observed band size: 221 kDa

false

  • Carrier free

    Anti-MYH7B antibody [EPR12290] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12290

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNP antibody [EPR20260-222] (AB309127)

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Primary Antibodies

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Western blot - Anti-Natriuretic peptides A antibody (AB180649)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Anti-TGF beta 1 antibody [EPR21143]

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Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified format use at 1/250 - 1/500. </strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in muscle contraction.
See full target information MYH7B
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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15017 PubMed40301504

2025

Protein interactions, network pharmacology, and machine learning work together to predict genes linked to mitochondrial dysfunction in hypertrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Lin Chen,Di Xiao,Yi-Jiang Liu,Zhan Wang,Zhi-Huang Chen,Rui Li,Li Li,Rong-Hai He,Shu-Yan Jiang,Xin Chen,Lin-Xi Xu,Feng-Chun Lu,Jia-Mao Wang,Zhong-Gui Shan

Communications biology 8:596 PubMed40210720

2025

The gut microbiota metabolite trimethylamine N-oxide promotes cardiac hypertrophy by activating the autophagic degradation of SERCA2a.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Lei,Yi Liu,Yuan Liu,Yujie Jiang,Yuyan Lei,Feilong Zhao,Wenqun Li,Zhonghua Ouyang,Lulu Chen,Siyuan Tang,Dongsheng Ouyang,Xiaohui Li,Ying Li

Molecular medicine reports 31: PubMed40183404

2025

α‑ketoglutarate protects against septic cardiomyopathy by improving mitochondrial mitophagy and fission.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wu,Qiong Ma,Bo-Tao Li,Shuang Shi,Gong-Chang Guan,Jun-Kui Wang,Bao-Yao Xue,Zhong-Wei Liu

British journal of pharmacology 182:2560-2581 PubMed39965654

2025

Imatinib aggravates pressure-overload-induced right ventricle failure via JNK/Runx2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Zeng,Zhuoji Ma,Shanshan Wen,Liang Zhou,Wanxian Hong,Zhixiong Wu,Chunxian Cen,Qianwen Bai,Shangwei Ding,Xin Chen,Jian Wang,Lingdan Chen,Wenju Lu,Tao Wang

International journal of molecular sciences 25: PubMed38542488

2024

Transcriptome and Metabolome Profiling Provide New Insights into Disuse Muscle Atrophy in Chicken: The Potential Role of Fast-Twitch Muscle Fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Zipei Yao,Lijin Guo,Li Zhang,Qinghua Nie

Signal transduction and targeted therapy 8:414 PubMed37857609

2023

Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wan,Zhen Zhang,Chennan Wu,Saisai Tian,Yibei Zang,Ge Jin,Qingyan Sun,Pin Wang,Xin Luan,Yili Yang,Xuelin Zhan,Lingyu Linda Ye,Dayue Darrel Duan,Xia Liu,Weidong Zhang

iScience 26:107826 PubMed37752946

2023

Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs.

Applications

Unspecified application

Species

Unspecified reactive species

Angelo M Lavecchia,Polyxeni Mantzouratou,Domenico Cerullo,Monica Locatelli,Sara Conti,Matteo Tironi,Fabio Sangalli,Daniela Corna,Carlamaria Zoja,Giuseppe Remuzzi,Christodoulos Xinaris

International journal of molecular sciences 24: PubMed37569807

2023

Inhibition of miR-25 Ameliorates Cardiac Dysfunction and Fibrosis by Restoring Krüppel-like Factor 4 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cholong Lee,Sunghye Cho,Dongtak Jeong

The Tohoku journal of experimental medicine 259:199-208 PubMed36517015

2022

MiR-423-5p Inhibition Exerts Protective Effects on Angiotensin II-Induced Cardiomyocyte Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Xu,Dongchen Liu,Xinyu Gao,Ziwen Wang,Linna Zhang,Hao Fan

Journal of the Endocrine Society 6:bvac132 PubMed36249410

2022

Castration of Male Mice Induces Metabolic Remodeling of the Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Elin Svedlund Eriksson,Inger Johansson,Anna K F Mårtensson,Marta Lantero Rodriguez,Maaike Schilperoort,Jan Kroon,Sander Kooijman,Elmir Omerovic,Linda Andersson,Malin C Levin,Patrick C N Rensen,Åsa Tivesten
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com