Name: Anti-MYH7B antibody [EPR12290]
SKU: ab172967
Rating: 4 (3 reviews)

Rabbit Recombinant Monoclonal MYH7B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 22 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (AB172967), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Tested
Mouse	Tested	Expected
Rat	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000 - 1/5000	Notes -
Species Rat	Dilution info 1/1000 - 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes For unpurified format use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information MYH7B

Function

Involved in muscle contraction.

Alternative names

KIAA1512, MYH7B, Myosin-7B, Antigen MLAA-21, Myosin cardiac muscle beta chain, Slow A MYH14

Recommended products

Rabbit Recombinant Monoclonal MYH7B antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 22 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR12290
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.05 µg/mL
All lanes: Human fetal muscle lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 221 kDa
Observed band size: 221 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling MYH7B with Purified ab172967 at 1:1000 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 0.1 µg/mL
Lane 1: Mouse heart lysate at 20 µg
Lane 2: Rat heart lysate at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 221 kDa
Observed band size: 221 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
ab172967 showing +ve staining in Human normal pancreas tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling MYH7B with ab172967 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
ab172967 showing -ve staining in Human normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYH7B antibody [EPR12290] (ab172967)
ab172967 showing -ve staining in Human normal breast tissue.
Western blot - Anti-MYH7B antibody [EPR12290] (ab172967)
All lanes: Western blot - Anti-MYH7B antibody [EPR12290] (ab172967) at 1/1000 dilution
Lane 1: Human fetal muscle lysates at 10 µg
Lane 2: Human fetal heart lysates at 10 µg
Lane 3: Human skeletal muscle lysates at 10 µg
Predicted band size: 221 kDa