Rabbit Polyclonal MYL12A phospho S19 antibody. Suitable for IP, ELISA, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Recombinant fragment, Mouse, Rat, Caenorhabditis elegans, Human, Recombinant full length protein samples. Cited in 144 publications. Immunogen corresponding to Synthetic Peptide within Human MYL12A phospho S19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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- Communications biology 6:81720233D cell segregation geometry and dynamics are governed by tissue surface tension regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElod Méhes,Enys Mones,Máté Varga,Áron Zsigmond,Beáta Biri-Kovács,László Nyitray,Vanessa Barone,Gabriel Krens,Carl-Philipp Heisenberg,Tamás VicsekPubMed 37542157
- Journal of biological engineering 17:422023Sandwich-like electro-conductive polyurethane-based gelatin/soybean oil nanofibrous scaffolds with a targeted release of simvastatin for cardiac tissue engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSolmaz Saghebasl,Abbas Nobakht,Hesam Saghebasl,Sanya Hayati,Ozra Naturi,Reza RahbarghaziPubMed 37415188
- Cell death & disease 14:3912023Internalization of apoptotic cells during efferocytosis requires Mertk-mediated calcium influx.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusumin Yang,Chanhyuk Min,Hyunji Moon,Byeongjin Moon,Juyeon Lee,Jaeseon Jeon,Hagyeong Kwon,Deokyun Jang,Daeho ParkPubMed 37391432
- European heart journal. Cardiovascular pharmacotherapy 9:371-3862023Vascular mechanisms of post-COVID-19 conditions: Rho-kinase is a novel target for therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert A Sykes,Karla B Neves,Rhéure Alves-Lopes,Ilaria Caputo,Kirsty Fallon,Nigel B Jamieson,Anna Kamdar,Assya Legrini,Holly Leslie,Alasdair McIntosh,Alex McConnachie,Andrew Morrow,Richard W McFarlane,Kenneth Mangion,John McAbney,Augusto C Montezano,Rhian M Touyz,Colin Wood,Colin BerryPubMed 37019821
- GeroScience 45:1471-14902023Longitudinal characterization of cerebral hemodynamics in the TgF344-AD rat model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Fang,Chengyun Tang,Huawei Zhang,Jane J Border,Yedan Liu,Seung Min Shin,Hongwei Yu,Richard J Roman,Fan FanPubMed 36933144
- Nature communications 14:3372023Wnt4 and ephrinB2 instruct apical constriction via Dishevelled and non-canonical signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaeho Yoon,Jian Sun,Moonsup Lee,Yoo-Seok Hwang,Ira O DaarPubMed 36670115
- Nature communications 14:822023An entosis-like process induces mitotic disruption in Pals1 microcephaly pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoelle A Sterling,Jun Young Park,Raehee Park,Seo-Hee Cho,Seonhee KimPubMed 36604424
- Journal of cellular physiology 239:e309382022Phosphoproteomic analysis identifies supervillin as an ERK3 substrate regulating cytokinesis and cell ploidy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoaquim Javary,Eugénie Goupil,Mathilde Soulez,Evgeny Kanshin,Antoine Bouchard,Ole-Morten Seternes,Pierre Thibault,Jean-Claude Labbé,Sylvain MelochePubMed 36576983
- iScience 25:1050502022Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaibin Tian,Zhiyang Chen,Xiaoman Zhu,Qingjian Ou,Zhe Wang,Binxin Wu,Jing-Ying Xu,Caixia Jin,Furong Gao,Juan Wang,Jingfa Zhang,Jieping Zhang,Lixia Lu,Guo-Tong XuPubMed 36185374
- International journal of molecular sciences 23:2022Dysregulation of the Enteric Nervous System in the Mid Colon of Complement Component 3 Knockout Mice with Constipation Phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Ju Choi,Hee Jin Song,Ji Eun Kim,Su Jin Lee,You Jeong Jin,Yu Jeong Roh,Ayun Seol,Hye Sung Kim,Dae Youn HwangPubMed 35743302
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human MYL12A phospho S19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P19105
Reactivity data
Select an application| IP | ELISA | WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Tested | Expected | Expected | Expected |
| Rat | Expected | Expected | Predicted | Expected | Predicted | Expected |
| Bird | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Caenorhabditis elegans | Expected | Expected | Predicted | Expected | Predicted | Expected |
| Mammals | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Recombinant fragment | Expected | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Mouse, Rat, Caenorhabditis elegans, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1/10000 - 1/30000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/30000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/30000 | Notes - |
Species Caenorhabditis elegans | Dilution info 1/10000 - 1/30000 | Notes - |
Species Human | Dilution info 1/10000 - 1/30000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info 1/500.00000 - 1/2000.00000 | Notes We recommend using BSA for blocking when using phospho-specific antibodies. |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes We recommend using BSA for blocking when using phospho-specific antibodies. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Bird, Mammals, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Trichloroacetic acid was used for tissue fixation. |
Species Rat | Dilution info - | Notes Trichloroacetic acid was used for tissue fixation. |
Species Caenorhabditis elegans | Dilution info - | Notes Trichloroacetic acid was used for tissue fixation. |
Species Human | Dilution info - | Notes Trichloroacetic acid was used for tissue fixation. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant full length protein | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Caenorhabditis elegans, Bird, Mammals, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant full length protein | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Caenorhabditis elegans, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Recombinant full length protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).
Targets
Myosin regulatory light chain 12A phospho S20
Alternative names
Myosin regulatory light chain 12A, Epididymis secretory protein Li 24, MLC-2B, Myosin RLC, Myosin regulatory light chain MRLC3, HEL-S-24, MRLC3, MLCB, RLC, MYL12A
Recommended products
Rabbit Polyclonal MYL12A phospho S19 antibody. Suitable for IP, ELISA, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Recombinant fragment, Mouse, Rat, Caenorhabditis elegans, Human, Recombinant full length protein samples. Cited in 144 publications. Immunogen corresponding to Synthetic Peptide within Human MYL12A phospho S19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human MYL12A phospho S19 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P19105
- Purification technique
Affinity purification Immunogen
- Specificity
ab2480 can detect the phosphorylated serine residue in both the human and mouse protein. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
This phospho specific polyclonal antibody is specific for phosphorylated pS19 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Customers have seen variable performance with Xenopus laevis.
- Concentration
- Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-MYL12A (phospho S19) antibody (ab2480)
All lanes: Western blot - Anti-MYL12A (phospho S19) antibody (ab2480) at 1/1000 dilution
Lane 1: Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
Lane 2: Regulatory Light Chain Phospho recombinant protein, 50 ng
Lane 3: Smooth Muscle Non-Phospho recombinant protein, 50 ng
Lane 4: Smooth Muscle Phospho recombinant protein, 50 ng
SecondaryAll lanes: Peroxidase rabbit secondary antibody at 1/40000 dilution
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12A (phospho S19) antibody (ab2480)
IHC-P of ab2480 at 2.5 μg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Western blot - Anti-MYL12A (phospho S19) antibody (ab2480)
Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA treated:
Lane 1 : untreated 45 min
Lane 2 : CLA 50 nm 45 min
Lane 3 : CLA 100 nm 45 min
Lane 4 : A21 untreated 45 min
Lane 5 : A22 A23187 5 min
Lane 6 : A23 A23187 15 min
Lane 7 : A24 A23187 60 min
After washing, a 1/5,000 dilution of anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090) was used as secondary.Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20
All lanes: Western blot - Anti-MYL12A (phospho S19) antibody (ab2480)
Predicted band size: 20 kDa
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