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AB137063

Anti-MYL12B antibody [EPR9331]

3

(1 Review)

|

(8 Publications)

Rabbit Recombinant Monoclonal MYL12B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

MRLC2, MYLC2B, MYL12B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, SHUJUN-1, MLC-2, MLC20

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (AB137063)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MYL12B antibody [EPR9331] (AB137063)

Immunofluorescent staining of Jurkat cells labelling MYL12B with ab137063 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (AB137063)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (AB137063)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MYL12B with ab137063 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (AB137063)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12B antibody [EPR9331] (AB137063)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MYL12B with ab137063 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MYL12B antibody [EPR9331] (AB137063)
  • WB

Unknown

Western blot - Anti-MYL12B antibody [EPR9331] (AB137063)

All lanes:

Western blot - Anti-MYL12B antibody [EPR9331] (ab137063) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Saos-2 cell lysate at 10 µg

Predicted band size: 20 kDa

false

  • Carrier free

    Anti-MYL12B antibody [EPR9331] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9331

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Clone EPR9331 is expected to react with MYL12A, MYL12B, MYL9A, and MYL9B

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Complementary Products

Primary Antibodies

AB79935

Anti-Myosin Light Chain 2 antibody

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Secondary Antibodies

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB2480

Anti-MYL12A (phospho S19) antibody

Western blot - Anti-MYL12A (phospho S19) antibody (AB2480)

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Primary Antibodies

AB209813

Anti-Cardiac Troponin T antibody [EPR20266]

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Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [EPR20266] (AB209813)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
See full target information MYL12B
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed35166236

2022

IP3 receptor orchestrates maladaptive vascular responses in heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Haikel Dridi,Gaetano Santulli,Jessica Gambardella,Stanislovas S Jankauskas,Qi Yuan,Jingyi Yang,Steven Reiken,Xujun Wang,Anetta Wronska,Xiaoping Liu,Alain Lacampagne,Andrew R Marks

Cell proliferation 54:e13041 PubMed33942933

2021

Therapeutic effects of CXCR4 subpopulation of transgene-free induced cardiosphere-derived cells on experimental myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyong Xu,Huimei Wu,Zhigang Mai,Junbo Yi,Xianqi Wang,Lingyun Li,Zhong Huang

JOR spine 4:e1146 PubMed34611583

2021

Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model.

Applications

Unspecified application

Species

Unspecified reactive species

Shangbin Cui,Zhiyu Zhou,Xu Chen,Fuxin Wei,R Geoff Richards,Mauro Alini,Sibylle Grad,Zhen Li

American journal of physiology. Cell physiology 315:C502-C515 PubMed29949406

2018

Transgelin induces dysfunction of fetal endothelial colony-forming cells from gestational diabetic pregnancies.

Applications

Unspecified application

Species

Unspecified reactive species

Kaela M Varberg,Rashell O Garretson,Emily K Blue,Chenghao Chu,Cassandra R Gohn,Wanzhu Tu,Laura S Haneline

International journal of molecular medicine 42:839-850 PubMed29786744

2018

miR‑133b regulates proliferation and apoptosis in high‑glucose‑induced human retinal endothelial cells by targeting ras homolog family member A.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yao,Jihong Wang,Yong Yao,Kelei Wang,Qianqian Zhou,Ying Tang

Experimental and therapeutic medicine 14:2221-2227 PubMed28962146

2017

Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yinyu Yin,Yiping Li,Jian Pan,Ruze Tang,Jie Zhu,Zhenfang Qin,Xiaobing Xu,Jian Wang

International journal of clinical and experimental 8:14409-16 PubMed26823757

2015

Identification of phosphorylated MYL12B as a potential plasma biomarker for septic acute kidney injury using a quantitative proteomic approach.

Applications

WB

Species

Mouse

Fan Wu,Xiu-Juan Dong,Yan-Yan Li,Yan Zhao,Qiu-Lin Xu,Lei Su

Cancer letters 364:142-55 PubMed25960282

2015

The development of cisplatin resistance in neuroblastoma is accompanied by epithelial to mesenchymal transition in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Piskareva,Harry Harvey,John Nolan,Ross Conlon,Leah Alcock,Patrick Buckley,Paul Dowling,Michael Henry,Finbarr O'Sullivan,Isabella Bray,Raymond L Stallings
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com