AB64161

Anti-MYL9 antibody

Name: Anti-MYL9 antibody (ab64161) | Abcam
Brand: Abcam Limited
SKU: AB64161
Price: 595 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal MYL9 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MYL9 aa 1-50.

View Alternative Names

MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (AB64161)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (AB64161)

ab64161 at 2.5μg/ml staining MYL9 in Colon, Human Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.

Unknown

Western blot - Anti-MYL9 antibody (AB64161)

All lanes:

Western blot - Anti-MYL9 antibody (ab64161) at 1/2000 dilution

All lanes:

Western blot - Recombinant Human MYL9 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-myl9-protein-ab104014'>ab104014</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 20 kDa

true

Exposure time: 4min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MYL9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P24844

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Bird": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed : 11942626, PubMed : 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

See full target information MYL9

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1168772 PubMed37251946

2023

Identification of the hub genes associated with prostate cancer tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Honghui Zhu,Qi Lin,Xiaomin Gao,Xixi Huang

Clinics and research in hepatology and gastroenter 44:101-114 PubMed31255531

2019

Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Kongxi Zhu,Yunxia Wang,Lan Liu,Shuai Li,Weihua Yu

Human molecular genetics 28:1429-1444 PubMed30566624

2018

Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Cho-Ming Chao,Alena Moiseenko,Djuro Kosanovic,Stefano Rivetti,Elie El Agha,Jochen Wilhelm,Marian Kampschulte,Faady Yahya,Harald Ehrhardt,Klaus-Peter Zimmer,Guillermo Barreto,Albert A Rizvanov,Ralph T Schermuly,Irwin Reiss,Rory E Morty,Robbert J Rottier,Saverio Bellusci,Jin-San Zhang

Journal of cellular physiology 233:8884-8895 PubMed29851084

2018

Elevated transforming growth factor β signaling activation in β-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xie,Piergiorgio Percipalle

Molecular human reproduction 18:265-79 PubMed22155728

2011

Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1).

Applications

Species

Unspecified reactive species

Claire A Hudson,Kate J Heesom,Andrés López Bernal

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYL9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (AB64161)

Western blot - Anti-MYL9 antibody (AB64161)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise