Rabbit Polyclonal MYL9 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MYL9 aa 1-50.
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Publications
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- Frontiers in oncology 13:11687722023Identification of the hub genes associated with prostate cancer tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHonghui Zhu,Qi Lin,Xiaomin Gao,Xixi HuangPubMed 37251946
- Clinics and research in hepatology and gastroenter 44:101-1142019Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKongxi Zhu et. al.PubMed 31255531
- Human molecular genetics 28:1429-14442018Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCho-Ming Chao,Alena Moiseenko,Djuro Kosanovic,Stefano Rivetti,Elie El Agha,Jochen Wilhelm,Marian Kampschulte,Faady Yahya,Harald Ehrhardt,Klaus-Peter Zimmer,Guillermo Barreto,Albert A Rizvanov,Ralph T Schermuly,Irwin Reiss,Rory E Morty,Robbert J Rottier,Saverio Bellusci,Jin-San ZhangPubMed 30566624
- Journal of cellular physiology 233:8884-88952018Elevated transforming growth factor β signaling activation in β-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Xie et. al.PubMed 29851084
- Molecular human reproduction 18:265-792011Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1).Applications:WBReactive species:Unspecified reactive speciesClaire A Hudson,Kate J Heesom,Andrés López BernalPubMed 22155728
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MYL9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P24844
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Bird | Predicted | Predicted |
| Mammals | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bird, Mammals | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).
Alternative names
MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20
Recommended products
Rabbit Polyclonal MYL9 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MYL9 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MYL9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P24844
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (ab64161)
ab64161 at 2.5μg/ml staining MYL9 in Colon, Human Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
Western blot - Anti-MYL9 antibody (ab64161)
All lanes: Western blot - Anti-MYL9 antibody (ab64161) at 1/2000 dilution
All lanes: Western blot - Recombinant Human MYL9 protein (Recombinant Human MYL9 protein ab104014) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Exposure time: 4min
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