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AB277790

Anti-MYL9 (phospho T18 + S19) antibody [18HCLC]

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Rabbit Recombinant Multiclonal MYL9 phospho S19 + T18 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human MYL9 phospho S19 + T18 aa 1-50.

View Alternative Names

MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (AB277790)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (AB277790)

For immunofluorescence analysis, C2C12 cells were fixed and permeabilized for detection of endogenous Myosin regulatory light polypeptide 9 (pT18/pS19) using Myosin regulatory light polypeptide 9 (pT18/pS19) Recombinant Rabbit Multiclonal Antibody (ab277790, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Myosin regulatory light polypeptide 9 (pT18/pS19) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Myosin regulatory light polypeptide 9 (pT18/pS19). Panel e) shows loss of signal by competition with the Myosin regulatory light polypeptide 9 (pT18/pS19) peptide, demonstrating antibody specificity, and panel f) demonstrates no competition with the non-phosphorylated peptide. Panel μ) represents control cells with no primary antibody to assess background.

Western blot - Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (AB277790)
  • WB

Supplier Data

Western blot - Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (AB277790)

All lanes:

Western blot - Anti-MYL9 (phospho T18 + S19) antibody [18HCLC] (ab277790) at 2 µg/mL

Lane 1:

C2C12 (Mouse myoblast cell line) whole cell extract

Lane 2:

C2C12 (Mouse myoblast cell line) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)

Lane 5:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract

Lane 6:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract (treated with Calyculin A 50 ng for 20 minutes)

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

18HCLC

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MYL9 phospho S19 + T18 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P24844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed : 11942626, PubMed : 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).
See full target information MYL9 phospho S19 + T18
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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