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Rabbit Recombinant Monoclonal MYL9 phospho S20 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Synthetic peptide - Human samples.

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Images

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (AB316750), expandable thumbnail
  • Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (AB316750), expandable thumbnail
  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (AB316750), expandable thumbnail
  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (AB316750), expandable thumbnail
  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (AB316750), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotICC/IFFlow Cyt (Intra)IPIHC-P
Human
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Target data

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

Additional Targets

MYL12A phospho S19, MYL12B phospho S20

Alternative names

MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20

Recommended products

Rabbit Recombinant Monoclonal MYL9 phospho S20 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27203-65
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750), expandable thumbnail

    Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750)

    MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) Western blot staining using rabbit Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 50 µg with 5% NFDM/TBST

    Lane 2: NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (untreated membrane) at 50 µg with 5% NFDM/TBST

    Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (alkaline phosphatase treated membrane) at 50 µg with 5% NFDM/TBST

    Lane 4: NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (alkaline phosphatase treated membrane) at 50 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 124 kDa

    Exposure time: 180s

  • Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750), expandable thumbnail

    Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750)

    MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) Dot Blot staining using rabbit Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody

    Dot blot analysis of MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) using ab316750 at 1:1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane 1: MYL9 (phospho S20) peptide

    Lane 2: MYL9 non-phospho peptide

    Lane 3: MYL9 (phospho S20 + T19) peptide

    Lane 4: MYL9 (phospho T19) peptide

    This antibody does not cross-react with human MYL9 (phospho T19) and MYL9 (phospho S20 + T19).

    All lanes: Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750) at 1/1000 dilution

    Lane 1: MYL9 (phospho S20) peptide

    Lane 2: MYL9 non-phospho peptide

    Lane 3: MYL9 (phospho S20 + T19) peptide

    Lane 4: MYL9 (phospho T19) peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750), expandable thumbnail

    Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (Anti-MYL9 antibody [EPR13012(2)] ab191393) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 (S20A mutant) expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 25 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750), expandable thumbnail

    Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750)

    The down-regulation of MYLK is induced by ML-7(PMID:28737169, PMID:9036856).

    The identity of the higher MW bands are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervical adenocarcinoma) treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate at 45 µg with 5% NFDM/TBST

    Lane 2: HeLa treated with 100ng/ml Calyculin A for 15 minutes then treated with 50uM ML7 for 15 minutes, whole cell lysate at 45 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 48s

  • Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750), expandable thumbnail

    Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750)

    The identity of the higher MW bands are unknown.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (Anti-MYL9 antibody [EPR13012(2)] ab191393) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (ab316750) at 1/1000 dilution

    Lane 1: Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (untreated membrane) at 45 µg with 5% NFDM/TBST

    Lane 2: HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (untreated membrane) at 45 µg with 5% NFDM/TBST

    Lane 3: Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (alkaline phosphatase treated membrane) at 45 µg with 5% NFDM/TBST

    Lane 4: HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 45 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa, 15 kDa

    Exposure time: 70s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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