AB316751

Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal MYL9 phospho S20 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Synthetic peptide - Human samples.

View Alternative Names

MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20, MLCB, MRLC3, RLC, MYL12A, Myosin regulatory light chain 12A, Epididymis secretory protein Li 24, MLC-2B, Myosin RLC, Myosin regulatory light chain MRLC3, HEL-S-24, MRLC2, MYLC2B, MYL12B, Myosin regulatory light chain 12B, MLC-2A, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, SHUJUN-1, MLC-2, MLC20

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Supplier Data

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

This data was developed using ab316750, the same antibody clone in a different buffer formulation.

The down-regulation of MYLK is induced by ML-7(PMID : 28737169, PMID : 9036856).

The identity of the higher MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (<a href='/en-us/products/primary-antibodies/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-ab316750'>ab316750</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervical adenocarcinoma) treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate at 45 µg

Lane 2:

HeLa treated with 100ng/ml Calyculin A for 15 minutes then treated with 50uM ML7 for 15 minutes, whole cell lysate at 45 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 48s

Supplier Data

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

This data was developed using ab316750, the same antibody clone in a different buffer formulation.

The identity of the higher MW bands are unknown.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (ab191393) staining at 1/1000 dilution.

All lanes:

Lane 1:

Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (untreated membrane) at 45 µg

Lane 2:

HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (untreated membrane) at 45 µg

Lane 3:

Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (alkaline phosphatase treated membrane) at 45 µg

Lane 4:

HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 45 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,15 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

This data was developed using ab316750, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 50 µg

Lane 2:

NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (untreated membrane) at 50 µg

Lane 3:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (alkaline phosphatase treated membrane) at 50 µg

Lane 4:

NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (alkaline phosphatase treated membrane) at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,124 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

This data was developed using ab316750, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (ab191393) staining at 1/1000 dilution.

All lanes:

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 (S20A mutant) expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

This data was developed using ab316750, the same antibody clone in a different buffer formulation.

Dot blot analysis of MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) using ab316750 at 1 : 1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1 : MYL9 (phospho S20) peptide
Lane 2 : MYL9 non-phospho peptide
Lane 3 : MYL9 (phospho S20 + T19) peptide
Lane 4 : MYL9 (phospho T19) peptide

This antibody does not cross-react with human MYL9 (phospho T19) and MYL9 (phospho S20 + T19).

All lanes:

Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (<a href='/en-us/products/primary-antibodies/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-ab316750'>ab316750</a>) at 1/1000 dilution

Lane 1:

MYL9 (phospho S20) peptide

Lane 2:

MYL9 non-phospho peptide

Lane 3:

MYL9 (phospho S20 + T19) peptide

Lane 4:

MYL9 (phospho T19) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Unconjugated

Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27203-65

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab316751 is the carrirer-free version of ab316750.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed : 11942626, PubMed : 2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

See full target information MYL9 pS20

Additional targets

MYL12A phospho S19,MYL12B phospho S20

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Product promise