Rabbit Recombinant Monoclonal MYL9 phospho S20 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Synthetic peptide - Human samples.
Images
![Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--dot-blot-img428244.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)")
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428225.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)")
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428226.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)")
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428224.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)")
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428223.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (AB316751)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Dot | ICC/IF | Flow Cyt (Intra) | IP | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate - Human, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).
Additional Targets
MYL12A phospho S19, MYL12B phospho S20
Alternative names
MLC2, MRLC1, MYRL2, MYL9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, MLC-2C, Myosin RLC, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, LC20
Recommended products
Rabbit Recombinant Monoclonal MYL9 phospho S20 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27203-65
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316751 is the carrirer-free version of Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--dot-blot-img428244.jpg "Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)")
Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)
MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) Dot Blot staining using rabbit Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody
This data was developed using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750, the same antibody clone in a different buffer formulation.
Dot blot analysis of MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750 at 1:1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lane 1: MYL9 (phospho S20) peptide
Lane 2: MYL9 non-phospho peptide
Lane 3: MYL9 (phospho S20 + T19) peptide
Lane 4: MYL9 (phospho T19) peptideThis antibody does not cross-react with human MYL9 (phospho T19) and MYL9 (phospho S20 + T19).
All lanes: Dot Blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750) at 1/1000 dilution
Lane 1: MYL9 (phospho S20) peptide
Lane 2: MYL9 non-phospho peptide
Lane 3: MYL9 (phospho S20 + T19) peptide
Lane 4: MYL9 (phospho T19) peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428225.jpg "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)")
Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)
MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) Western blot staining using rabbit Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody
This data was developed using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 50 µg with 5% NFDM/TBST
Lane 2: NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (untreated membrane) at 50 µg with 5% NFDM/TBST
Lane 3: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (alkaline phosphatase treated membrane) at 50 µg with 5% NFDM/TBST
Lane 4: NIH/3T3 treated with 100nM Calycin A for 30min whole cell lysateug (alkaline phosphatase treated membrane) at 50 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20 kDa, 124 kDa
Exposure time: 180s
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428226.jpg "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)")
Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)
This data was developed using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (Anti-MYL9 antibody [EPR13012(2)] ab191393) staining at 1/1000 dilution.
All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T (human embryonic kidney epithelial cell) cells transfected with human MYL9 (S20A mutant) expression vector containing a myc-His-tag® then treated with 100/ml calyculin for 15 minutes, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428224.jpg "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)")
Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)
This data was developed using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750, the same antibody clone in a different buffer formulation.
The down-regulation of MYLK is induced by ML-7(PMID:28737169, PMID:9036856).
The identity of the higher MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervical adenocarcinoma) treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate at 45 µg with 5% NFDM/TBST
Lane 2: HeLa treated with 100ng/ml Calyculin A for 15 minutes then treated with 50uM ML7 for 15 minutes, whole cell lysate at 45 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 36 kDa
Exposure time: 48s
![Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751), expandable thumbnail](https://content.abcam.com/products/images/myl9-ps20-myl12a-ps19-myl12b-ps20-antibody-epr27203-65-bsa-and-azide-free-ab316751--western-blot-img428223.jpg "Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)")
Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] - BSA and Azide free (ab316751)
This data was developed using Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750, the same antibody clone in a different buffer formulation.
The identity of the higher MW bands are unknown.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-MYL9 antibody [EPR13012 (2)] (Anti-MYL9 antibody [EPR13012(2)] ab191393) staining at 1/1000 dilution.
All lanes: Western blot - Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] (Anti-MYL9 (pS20) + MYL12A (pS19) + MYL12B (pS20) antibody [EPR27203-65] ab316750) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (untreated membrane) at 45 µg with 5% NFDM/TBST
Lane 2: HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (untreated membrane) at 45 µg with 5% NFDM/TBST
Lane 3: Untreated HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate (alkaline phosphatase treated membrane) at 45 µg with 5% NFDM/TBST
Lane 4: HeLa treated with 100ng/ml Calyculin A for 15 minutes, whole cell lysate (alkaline phosphatase treated membrane) at 45 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 20 kDa, 15 kDa
Exposure time: 70s
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