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AB214313

Anti-MYLPF antibody

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(1 Publication)

Rabbit Polyclonal MYLPF antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MYL11 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HSRLC, MYLPF, MYL11, Myosin regulatory light chain 11, Fast skeletal myosin light chain 2, MLC2B, Myosin light chain 11

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYLPF antibody (AB214313)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYLPF antibody (AB214313)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse embryonic muscle tissue labeling MYLPF with ab214313 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-MYLPF antibody (AB214313)
  • WB

Lab

Western blot - Anti-MYLPF antibody (AB214313)

All lanes:

Western blot - Anti-MYLPF antibody (ab214313) at 1/1000 dilution

All lanes:

Mouse muscle lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MYL11 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96A32

Specificity

ab214313 may recognize myosin light chain 5, myosin light chain 7, or myosin regulatory light chain 10.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYLPF also known as Myosin Light Chain Phosphorylatable Fast Skeletal Muscle is a protein with a molecular mass of approximately 25 kDa. This protein plays an important role in muscle contraction by regulating the actin-myosin interactions in fast skeletal muscles. It is primarily found in the skeletal muscle tissue and has a critical function in modulating the contractile properties of these muscles. MYLPF belongs to the myosin light chain family and interacts closely within the sarcomere the basic unit of muscle fiber.
Biological function summary

MYLPF influences muscle function by participating in the regulation of sarcomere structure and muscle contraction dynamics. It functions in a complex with other myosin light chains acting as a regulator in muscle fiber tension and contraction rates. MYLPF specifically affects the phosphorylation state of the myosin light chain which alters muscle contraction speed and efficiency. The protein balances muscle force generation and plays an essential role in fine-tuning motor activity especially in rapid and powerful movements.

Pathways

MYLPF is an integral component in signaling that controls muscle contraction and relaxation processes. It is tightly associated with the calcium signaling pathway which regulates muscle contraction in response to neural stimuli. Additionally MYLPF interacts with other proteins within the sarcomere such as myosin heavy chains and collaborates with calcium-calmodulin-dependent protein kinases. These interactions are pivotal for transducing signals essential for muscle function contraction timing and coordination.

Alterations in MYLPF expression or function have links to muscular dystrophies which are characterized by progressive weakness and loss of muscle mass. Dysregulation of MYLPF may also contribute to cardiac disorders impacting heart muscle function and leading to cardiomyopathies. The close interaction of MYLPF with proteins like myosin heavy chains in muscle fibers denotes a possible pathway through which mutations or dysregulations propagate muscle-related diseases. Understanding MYLPF's role in these pathologies offers insight into potential therapeutic strategies aimed at muscle function restoration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosin regulatory subunit that plays an essential role to maintain muscle integrity during early development (By similarity). Plays a role in muscle contraction (By similarity).
See full target information MYL11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113112 PubMed40792031

2025

A long-lasting prolactin stimulates galactopoiesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kasia Kready,Kailyn E Doiron,Katherine Redfield Chan,Jeffrey C Way,Quincey Justman,Camille E Powe,Pamela A Silver
View all publications
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