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AB224120

Anti-MYO10 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal MYO10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MYO10 aa 900-1050.

View Alternative Names

KIAA0799, MYO10, Unconventional myosin-X, Unconventional myosin-10

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-MYO10 antibody (AB224120)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MYO10 antibody (AB224120)

Immunofluorescent staining with ab224120 (green) of human cell line HeLa shows localization to plasma membrane and the tips of filopodia.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MYO10 aa 900-1050. The exact immunogen used to generate this antibody is proprietary information.

Q9HD67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mechanically MYO10 also known as Myosin-X is an unconventional myosin motor protein with a molecular mass of approximately 237 kDa. This protein has a significant presence in specialized cellular structures such as filopodia and neuron growth cones. It functions as a motor that transports cellular cargo along actin filaments playing an important role in cellular movement and organization. Expression of MYO10 occurs in various tissues but it is notably abundant in the brain and testis.
Biological function summary

MYO10 facilitates several cellular processes by mediating actin-based transport and cellular structure dynamics. It forms part of a complex network interacting with actin filaments influencing the dynamics of cell protrusions such as filopodia. These processes are essential for cell migration development and intracellular transport where MYO10 acts as an anchor enabling cells to respond to external stimuli and migrate accordingly.

Pathways

MYO10 interacts with various molecular networks influencing critical signaling cascades. It plays a significant role in the Wnt signaling pathway by interacting with Dishevelled (Dvl) proteins impacting cell polarization and movement. Additionally MYO10 is involved in integrin signaling facilitating the formation of adhesive contacts necessary for cell movement linking proteins such as talin and integrins into the pathway for integrative structural and signal modulation.

MYO10 has connections with certain pathologies particularly cancer and neurological disorders. Abnormal expression or mutation of MYO10 could contribute to cancer progression by disrupting normal cell migration and adhesion thereby aiding metastasis. In neurological disorders MYO10 has associations with proteins such as Reelin and its dysregulation could contribute to impaired neuronal migration or connectivity elucidating some neurological pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as a plus end-directed motor. Moves with higher velocity and takes larger steps on actin bundles than on single actin filaments (PubMed : 27580874). The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts.. Isoform Headless. Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion (By similarity).
See full target information MYO10
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

microPublication biology 2025: PubMed41050330

2025

Cells stably expressing shRNA against MYO10 display altered cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna A Mas,Chase E Cristella,Vu Hao M N Phan,Lillian S Wendt,Charlotte A Rose,Abigail Ali,David F Carpio,Christine Cole,Paige Embley,Jack E Hoskins-Harris,Delia Johnson,Noelle Ledoux,Hannah W Lwin,Sarah Salah,Erin Weisbart,Stacey J Criswell,Omar Alberto Quintero-Carmona

Nature immunology 26:42-52 PubMed39633178

2024

Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline L Holley,Mercedes Monteleone,Daniel Fisch,Alexandre E S Libert,Robert J Ju,Joon H Choi,Nicholas D Condon,Stefan Emming,Joanna Crawford,Grace M E P Lawrence,Jared R Coombs,James G Lefevre,Rinie Bajracharya,Mireille H Lahoud,Alpha S Yap,Nicholas Hamilton,Samantha J Stehbens,Jonathan C Kagan,Nicholas Ariotti,Sabrina S Burgener,Kate Schroder

Acta pharmacologica Sinica 45:193-208 PubMed37749237

2023

Rosmarinic acid in combination with ginsenoside Rg1 suppresses colon cancer metastasis via co-inhition of COX-2 and PD1/PD-L1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Rui Deng,Cheng-Wei Zhu,Hong-Kuan Han,Gang-Fan Zong,Lang Ren,Peng Cheng,Zhong-Hong Wei,Yang Zhao,Su-Yun Yu,Yin Lu

Journal of cardiothoracic surgery 18:222 PubMed37420297

2023

Higher expression of pseudouridine synthase 7 promotes non-small cell lung cancer progression and suggests a poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Zhang,Yongde Zhu,Yonghuang Tan,Biao Chen,Shichao Shan,Gengyu Zhang,Jianjun Lu
View all publications
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Product promise

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