AB327127

Anti-MYO10 Antibody [CL9032]

Name: Anti-MYO10 Antibody [CL9032] (ab327127) | Abcam
Brand: Abcam Limited
SKU: AB327127
Price: 600 USD
Availability: InStock

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Mouse Monoclonal MYO10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MYO10.

View Alternative Names

KIAA0799, MYO10, Unconventional myosin-X, Unconventional myosin-10

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO10 Antibody [CL9032] (AB327127)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO10 Antibody [CL9032] (AB327127)

Immunohistochemical staining of human testis using ab327127 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in Leydig cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO10 Antibody [CL9032] (AB327127)

Immunohistochemical staining of human placenta using ab327127 at 1 : 200–1 : 500 dilution, showing moderate cytoplasmic positivity in trophoblastic cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO10 Antibody [CL9032] (AB327127)

Immunohistochemical staining of human cerebral cortex using ab327127 at 1 : 200–1 : 500 dilution, showing strong cytoplasmic positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO10 Antibody [CL9032] (AB327127)

Immunohistochemical staining of human skeletal muscle using ab327127 at 1 : 200–1 : 500 dilution, showing very weak cytoplasmic positivity in myocytes as expected.

Supplier Data

Western blot - Anti-MYO10 Antibody [CL9032] (AB327127)

Western blot analysis in human cell line SK-MEL-30 using ab327127 at 1 µg/ml.

All lanes:

Western blot - Anti-MYO10 Antibody [CL9032] (ab327127)

All lanes:

Human cell line SK-MEL-30

Predicted band size: 237.35 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL9032

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MYO10.

Q9HD67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as a plus end-directed motor. Moves with higher velocity and takes larger steps on actin bundles than on single actin filaments (PubMed : 27580874). The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts.. Isoform Headless. Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion (By similarity).

See full target information MYO10

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Product promise

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