AB174286

Anti-MYO19 antibody [EPR12551-13]

Name: Anti-MYO19 antibody [EPR12551-13] (ab174286) | Abcam
Brand: Abcam Limited
SKU: AB174286
Price: 365 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal MYO19 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

MYOHD1, MYO19, Unconventional myosin-XIX, Myosin head domain-containing protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MYO19 antibody [EPR12551-13] (AB174286)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYO19 antibody [EPR12551-13] (AB174286)

Immunofluorescent analysis of A549 cells labeling MYO19 with ab174286 at 1/50 (red). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-MYO19 antibody [EPR12551-13] (AB174286)

All lanes:

Western blot - Anti-MYO19 antibody [EPR12551-13] (ab174286) at 1/1000 dilution

Lane 1:

HeLa cell line lysate at 10 µg

Lane 2:

A549 cell line lysate at 10 µg

Lane 3:

K562 cell line lysate at 10 µg

Lane 4:

293T cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 109 kDa

true

Carrier free

Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12551-13

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MYO19 protein also known as Myosin XIX functions as an ATP-dependent motor protein. It possesses a molecular mass of approximately 119 kDa and is part of the myosin superfamily. This protein is known for its role in cellular processes requiring motility including vesicle and organelle transport. MYO19 is widely expressed in tissues with notable expression in the brain and skeletal muscles suggesting its importance in those regions.

Biological function summary

MYO19 contributes to mitochondrial dynamics by participating in mitochondrial motility and positioning within cells. While MYO19 is not part of a larger protein complex it interacts with the mitochondrial outer membrane and aids in tethering mitochondria to the cytoskeleton. This interaction is essential for maintaining proper cellular function specifically in energy-demanding tissues.

Pathways

MYO19 interacts with cellular transport mechanisms notably involving the actin cytoskeleton pathway. The protein facilitates mitochondrial movement along actin filaments. It shares pathways with other myosin family members such as MYO5 which are involved in intracellular transport. Additionally MYO19's role in actin-related pathways underlines its involvement in regulating cellular energy distribution and homeostasis.

MYO19 has potential links with neurodegenerative diseases and muscular disorders. Disruption in MYO19 function may lead to impaired mitochondrial distribution contributing to the pathology of conditions like amyotrophic lateral sclerosis (ALS). Additionally the protein shows possible connections with neurofilament-associated proteins suggesting a role in neural health. Such associations highlight the significance of MYO19 in understanding disease mechanisms and designing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed : 19932026, PubMed : 23568824, PubMed : 25447992). Motor protein that moves towards the plus-end of actin filaments (By similarity). Required for mitochondrial inheritance during mitosis (PubMed : 25447992). May be involved in mitochondrial transport or positioning (PubMed : 23568824).

See full target information MYO19

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:765 PubMed40830974

2025

Coordinating oncogenesis and immune evasion: KPNA2, GOLM1, and TK1 as novel CAR T-cell targets in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wangrui Liu,Hui Zou,Lei Guo,Zhonghua Zhou,Yahui Xie,Huaqi Guo,Gang Wei,Kai Zhang,Hui Yin,Shiyin Wei,Jiachang Chi

Journal of cell science 136: PubMed36744380

2023

Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Coscia,Cameron P Thompson,Qing Tang,Elana E Baltrusaitis,Joseph A Rhodenhiser,Omar A Quintero-Carmona,E Michael Ostap,Melike Lakadamyali,Erika L F Holzbaur

Journal of cell science 134: PubMed34013964

2021

Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Majstrowicz,Ulrike Honnert,Petra Nikolaus,Vera Schwarz,Stefanie J Oeding,Sandra A Hemkemeyer,Martin Bähler

Nature 591:659-664 PubMed33658713

2021

Actin cables and comet tails organize mitochondrial networks in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Moore,Stephen M Coscia,Cory L Simpson,Fabian E Ortega,Eric C Wait,John M Heddleston,Jeffrey J Nirschl,Christopher J Obara,Pedro Guedes-Dias,C Alexander Boecker,Teng-Leong Chew,Julie A Theriot,Jennifer Lippincott-Schwartz,Erika L F Holzbaur

Journal of cell science 131: PubMed30111583

2018

Identification of Miro1 and Miro2 as mitochondrial receptors for myosin XIX.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie J Oeding,Katarzyna Majstrowicz,Xiao-Ping Hu,Vera Schwarz,Angelika Freitag,Ulrike Honnert,Petra Nikolaus,Martin Bähler

Scientific reports 7:11577 PubMed28912530

2017

ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain.

Applications

Unspecified application

Species

Unspecified reactive species

Boris I Shneyer,Marko Ušaj,Naama Wiesel-Motiuk,Ronit Regev,Arnon Henn

Journal of cell science 129:543-56 PubMed26659663

2015

Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia.

Applications

Unspecified application

Species

Unspecified reactive species

Boris I Shneyer,Marko Ušaj,Arnon Henn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYO19 antibody [EPR12551-13]

Immunocytochemistry/ Immunofluorescence - Anti-MYO19 antibody [EPR12551-13] (AB174286)

Western blot - Anti-MYO19 antibody [EPR12551-13] (AB174286)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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