Rabbit Polyclonal MYO1A antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MYO1A aa 250-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MYO1A aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBC5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Involved in directing the movement of organelles along actin filaments.
Alternative names
MYHL, MYO1A, Unconventional myosin-Ia, Brush border myosin I, Myosin I heavy chain, BBM-I, BBMI, MIHC
Recommended products
Rabbit Polyclonal MYO1A antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MYO1A aa 250-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MYO1A aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBC5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MYO1A also known as Myosin IA is a non-muscle myosin protein. It weighs approximately 130 kDa. This protein functions as an actin-based motor molecule that uses ATP hydrolysis for movement and plays a role in cellular processes such as membrane trafficking and maintaining cell shape. MYO1A is expressed in several tissues most abundantly in the intestinal epithelial cells where it is critical for the distribution of microvilli and transporting cargo along the actin filaments.
- Biological function summary
MYO1A contributes to intestinal epithelial integrity and brush border assembly as a part of the cytoskeleton. It operates in concert with other actin-binding proteins to stabilize the microvillar structure and is not a part of a larger complex. By coupling actin dynamics with cellular signaling MYO1A facilitates the organization and renewal of the apical structures in epithelial cells therefore supporting vital transport and barrier functions.
- Pathways
MYO1A plays a significant role in the cytoskeleton organization pathway and the transport of molecules in epithelial cells. It interacts with actin filaments and is functionally related to proteins such as EPS8 which regulates actin dynamics contributing to cell shape modulation and signal transduction. Its involvement in these pathways enables coordinated actions essential for cellular homeostasis in epithelial tissues.
- Associated diseases and disorders
MYO1A mutations are linked with hearing loss indicating its importance in auditory mechanisms. It also relates to colorectal cancer due to its role in maintaining cell polarity and structure in epithelial tissues. In the context of these diseases MYO1A’s interaction with other proteins such as actin and its coordinating regulators like EPS8 may affect cellular functions leading to disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-MYO1A antibody (ab154518)
5% SDS PAGE
All lanes: Western blot - Anti-MYO1A antibody (ab154518) at 1/2000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: K562 whole cell lysate at 30 µg
Lane 4: NCI H929 whole cell lysate at 30 µg
Predicted band size: 118 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1A antibody (ab154518)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma, labeling MYO1A using ab154518 at 1/500 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com