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AB240334

Anti-MYO1B antibody [EPR16223] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MYO1B antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Unconventional myosin-Ib, MYH-1c, Myosin I alpha, MMI-alpha, MMIa, MYO1B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1B antibody [EPR16223] - BSA and Azide free (AB240334)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1B antibody [EPR16223] - BSA and Azide free (AB240334)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MYO1B with ab194356 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194356).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1B antibody [EPR16223] - BSA and Azide free (AB240334)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1B antibody [EPR16223] - BSA and Azide free (AB240334)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MYO1B with ab194356 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194356).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16223

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab240334 is the carrier-free version of ab194356.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYO1B also known as Myosin IB belongs to the myosin superfamily. It weighs approximately 123 kDa. MYO1B functions as an actin-binding protein with ATPase activity contributing to cellular processes like membrane tension and transport. This protein is expressed in various tissues including brain intestines and kidneys. It plays a role in dynamic cellular environments where it influences membrane dynamics and trafficking.
Biological function summary

MYO1B participates in cellular processes by associating with actin filaments. MYO1B contributes to vesicular transport and cell cortex regulation. It acts as a monomer and does not form complexes with other myosins. MYO1B's ability to interact with plasma membrane and microfilaments facilitates movement and stabilization tasks essential for maintaining cellular structure and function.

Pathways

MYO1B interacts with dynamic cellular events involving actin remodeling and intracellular transport. The protein plays roles in the actin cytoskeleton pathway and is linked to the regulation of endocytosis. MYO1B interacts with proteins like actin and other myosins which support its functions within the pathways and ensure effective cellular responses to environmental cues.

Researchers have associated MYO1B with conditions like deafness and some cancers. The protein's influence on cell membrane dynamics and movement may link it to hearing-related disorders. Its dysregulation or alterations can affect cellular behavior contributing to tumorigenic pathways. In cancer research MYO1B interacts with proteins such as cadherins showing potential pathways that can be important in disease progression and therapy targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Motor protein that may participate in process critical to neuronal development and function such as cell migration, neurite outgrowth and vesicular transport.
See full target information Unconventional myosin-Ib
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105723 PubMed36590169

2023

Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips.

Applications

Unspecified application

Species

Unspecified reactive species

Chuntao Wu,Beiyuan Hu,Lei Wang,Xia Wu,Haitao Gu,Hanguang Dong,Jiuliang Yan,Zihao Qi,Qi Zhang,Huan Chen,Bo Yu,Sheng Hu,Yu Qian,Shuang Dong,Qiang Li,Xu Wang,Jiang Long
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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