Anti-MYO1C antibody [EPR14771] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (AB251196)

This data was developed using ab194828, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labelling MYO1C (red) with purified ab194828 at dilution of 1/80. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (AB251196)

This data was developed using ab194828, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A431 cells labeling MYO1C with ab194828 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm and nuclear on A431 cell line was observed.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (AB251196)

This data was developed using ab194828, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYO1C with ab194828 at 1/200 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab194828. Cytoplasm staining on Human tonsil was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (AB251196)

This data was developed using ab194828, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MYO1C antibody [EPR14771] (<a href='/en-us/products/primary-antibodies/myo1c-antibody-epr14771-ab194828'>ab194828</a>) at 1/20000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

HT1080 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa

false

• WB

Supplier Data

Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (AB251196)

This data was developed using ab194828, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MYO1C antibody [EPR14771] (<a href='/en-us/products/primary-antibodies/myo1c-antibody-epr14771-ab194828'>ab194828</a>) at 1/2000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa

false