AB235003

Anti-MYO5A antibody

Name: Anti-MYO5A antibody (ab235003) | Abcam
Brand: Abcam Limited
SKU: AB235003
Price: 525 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal MYO5A antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MYO5A aa 1050-1250.

View Alternative Names

MYH12, MYO5A, Unconventional myosin-Va, Myosin heavy chain 12, Myosin-12, Myoxin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5A antibody (AB235003)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5A antibody (AB235003)

Paraffin-embedded human brain tissue stained for MYO5A using ab235003 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temeprature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

Supplier Data

Western blot - Anti-MYO5A antibody (AB235003)

All lanes:

Western blot - Anti-MYO5A antibody (ab235003) at 1/500 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 105 kDa,109 kDa,115 kDa,215 kDa,45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MYO5A aa 1050-1250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4I1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin Va also known as MYO5A is a motor protein with a mass of approximately 220 kDa. It plays an important role in intracellular transport by moving along actin filaments. This protein has two motor heads that use ATP hydrolysis to generate force for movement. MYO5A is widely expressed in a variety of tissues including the brain melanocytes and other neuronal tissues. It is vital for processes such as organelle transport vesicle trafficking and the distribution of melanin.

Biological function summary

This protein acts as a cargo transporter within cells moving materials to specific destinations. It can form a complex with Rab27A aiding in the transport of organelles like melanosomes and synaptic vesicles. In neurons MYO5A contributes to synaptic function by ensuring the proper localization of neurotransmitter-containing vesicles. Its role in pigment-producing cells is critical for the even distribution of melanin which is important for skin and hair color.

Pathways

MYO5A participates in the melanosome transport pathway and the neuroplasticity pathway. In the melanosome transport pathway it collaborates with the Rab27A and melanophilin proteins to ensure proper melanosome localization within melanocytes. This pathway is important for pigmentation. In the context of neuroplasticity MYO5A interacts with proteins that influence synaptic plasticity and vesicle trafficking such as synaptophysin and synapsin to support efficient synaptic signaling.

Abnormalities in MYO5A are associated with Griscelli syndrome type 1 and neurological disorders. Griscelli syndrome type 1 involves defects in the MYO5A protein disrupting melanosome transport and leading to pigmentary dilution. In neurological disorders the protein's connection with synaptic function makes it relevant for conditions linked to impaired neurotransmitter distribution. MYO5A's interaction with Rab27A is important for both pigmentation and proper synaptic vesicle transport tying it to the pathological features observed in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.

See full target information MYO5A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29643 PubMed40804093

2025

miR-221 activates Sox11 to reduce brain injury after intracerebral hemorrhage via inhibiting neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Liang,Yongchun Liu,Renyang Liu,Xianmei Shen,Mingxia Xu

Experimental dermatology 31:1253-1263 PubMed35514241

2022

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjin Wei,Min Huang,Yi Yang,Yali Liu,Sumin Chi,Chengxin Li

FEBS open bio 10:1891-1899 PubMed32735728

2020

Identification of three predictors of gastric cancer progression and prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Shuhua Chen,Rongzhang Xie,Pengpeng Jiang,Changjun Yu,Jinmei Fang,Xingcun Liu,Fazhi Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYO5A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5A antibody (AB235003)

Western blot - Anti-MYO5A antibody (AB235003)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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