Rabbit Recombinant Monoclonal MYO5B antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human samples.
Images
![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419342.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796)")
![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419343.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796)")
![Dot Blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--dot-blot-img419447.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796)")
![Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--immunocytochemistry-immunofluorescence-img419411.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796)")
![Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--immunocytochemistry-immunofluorescence-img419410.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (AB313796)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | WB | Dot | IHC-P | IHC-Fr | IP | |
|---|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis. Required for proper localization of bile salt export pump ABCB11 at the apical/canalicular plasma membrane of hepatocytes (PubMed:34816459).
Alternative names
KIAA1119, MYO5B, Unconventional myosin-Vb
Recommended products
Rabbit Recombinant Monoclonal MYO5B antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28275-55
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human FC-Intra and ICC/IF.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MYO5B also known as myosin-Vb is a motor protein that plays a significant role in cellular transport. It exhibits a molecular mass of approximately 215 kDa. MYO5B interacts with actin filaments conducting transport of cargo through the cell via ATP hydrolysis. It is chiefly found in epithelial cells such as those lining the intestines and kidneys. This protein localizes to various intracellular compartments including the plasma membrane and endosomal compartments facilitating organelle and protein transport critical for maintaining cellular function.
- Biological function summary
The mechanical movements generated by MYO5B are essential for intracellular trafficking processes. It serves as a component of the transport machinery involving membrane trafficking ensuring the proper distribution of cellular materials. As part of a molecular complex MYO5B works in conjunction with Rab GTPases to regulate vesicle movement. This coordination helps maintain the polarity and integrity of epithelial cells which are important for nutrient absorption and secretion in tissues.
- Pathways
MYO5B is intimately involved in the regulation of the endocytic recycling pathway. It plays a pivotal role in the recycling of receptors and lipids back to the plasma membrane. The protein interfaces with key elements such as RAB11A which is important for the recycling pathway. MYO5B’s function in this pathway is integral for maintaining epithelial cell dynamics and tissue homeostasis ensuring correct receptor and nutrient transporter localization in the cellular membrane.
- Associated diseases and disorders
Dysfunctional MYO5B is implicated in microvillus inclusion disease (MVID) a disorder that disrupts nutrient absorption in the intestines. This protein forms links with RAB11A and promotes disruptions when mutated leading to the symptoms observed in MVID. Additionally MYO5B mutations have associations with cholestasis a condition where bile cannot flow from the liver to the duodenum. The relationship with other proteins like syntaxin 3 further complicates cholestasis pathophysiology highlighting MYO5B’s broad impact in human diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419342.jpg "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-MYO5B antibody [EPR28275-55] (Anti-MYO5B antibody [EPR28275-55] ab313795) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 30 µg
Lane 2: Human skeletal muscle tissue lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 213 kDa
Exposure time: 180s
![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419343.jpg "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-MYO5B antibody [EPR28275-55] (Anti-MYO5B antibody [EPR28275-55] ab313795) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 30 µg
Lane 2: Mouse skeletal muscle tissue lysate at 30 µg
Lane 3: Rat brain tissue lysate at 30 µg
Lane 4: Rat skeletal muscle tissue lysate at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 213 kDa
Exposure time: 180s
![Dot Blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--dot-blot-img419447.jpg "Dot Blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Dot Blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Dot blot analysis of MYO5B using Anti-MYO5B antibody [EPR28275-55] ab313795 at 1:1000 (0.527 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: MYO5B peptide
Lane 2: MYO5A peptide
Lane 3: MYO5C peptide
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST![Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--immunocytochemistry-immunofluorescence-img419411.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized rat primary neuron cells labelling MYO5B with Anti-MYO5B antibody [EPR28275-55] ab313795 at 1/50 (10.54 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--immunocytochemistry-immunofluorescence-img419410.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Immunocytochemistry/ Immunofluorescence - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling MYO5B with Anti-MYO5B antibody [EPR28275-55] ab313795 at 1/50 (10.54 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419341.jpg "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: U-937, THP-1.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 secondsAll lanes: Western blot - Anti-MYO5B antibody [EPR28275-55] (Anti-MYO5B antibody [EPR28275-55] ab313795) at 1/1000 dilution
Lane 1: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 30 µg
Lane 2: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 30 µg
Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 213 kDa
Exposure time: 59s
![Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--flow-cytometry-intracellular-img419422.jpg "Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling MYO5B with Anti-MYO5B antibody [EPR28275-55] ab313795 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--flow-cytometry-intracellular-img419421.jpg "Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)
This data was developed using Anti-MYO5B antibody [EPR28275-55] ab313795, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling MYO5B with Anti-MYO5B antibody [EPR28275-55] ab313795 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--western-blot-img419341.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
![Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--flow-cytometry-intracellular-img419422.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
![Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796), expandable thumbnail](https://content.abcam.com/products/images/myo5b-antibody-epr28275-55-bsa-and-azide-free-ab313796--flow-cytometry-intracellular-img419421.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MYO5B antibody [EPR28275-55] - BSA and Azide free (ab313796)")
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