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AB190096

Anti-MYO5B antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal MYO5B antibody. N-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MYO5B.

View Alternative Names

KIAA1119, MYO5B, Unconventional myosin-Vb

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5B antibody - N-terminal (AB190096)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5B antibody - N-terminal (AB190096)

Immunohistochemical analysis of formalin fixed paraffin embedded human skeletal muscle labeling MYO5B with ab190096 at 5 μg/ml

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MYO5B. The exact immunogen used to generate this antibody is proprietary information.

Q9ULV0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYO5B also known as myosin-Vb is a motor protein that plays a significant role in cellular transport. It exhibits a molecular mass of approximately 215 kDa. MYO5B interacts with actin filaments conducting transport of cargo through the cell via ATP hydrolysis. It is chiefly found in epithelial cells such as those lining the intestines and kidneys. This protein localizes to various intracellular compartments including the plasma membrane and endosomal compartments facilitating organelle and protein transport critical for maintaining cellular function.
Biological function summary

The mechanical movements generated by MYO5B are essential for intracellular trafficking processes. It serves as a component of the transport machinery involving membrane trafficking ensuring the proper distribution of cellular materials. As part of a molecular complex MYO5B works in conjunction with Rab GTPases to regulate vesicle movement. This coordination helps maintain the polarity and integrity of epithelial cells which are important for nutrient absorption and secretion in tissues.

Pathways

MYO5B is intimately involved in the regulation of the endocytic recycling pathway. It plays a pivotal role in the recycling of receptors and lipids back to the plasma membrane. The protein interfaces with key elements such as RAB11A which is important for the recycling pathway. MYO5B’s function in this pathway is integral for maintaining epithelial cell dynamics and tissue homeostasis ensuring correct receptor and nutrient transporter localization in the cellular membrane.

Dysfunctional MYO5B is implicated in microvillus inclusion disease (MVID) a disorder that disrupts nutrient absorption in the intestines. This protein forms links with RAB11A and promotes disruptions when mutated leading to the symptoms observed in MVID. Additionally MYO5B mutations have associations with cholestasis a condition where bile cannot flow from the liver to the duodenum. The relationship with other proteins like syntaxin 3 further complicates cholestasis pathophysiology highlighting MYO5B’s broad impact in human diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis. Required for proper localization of bile salt export pump ABCB11 at the apical/canalicular plasma membrane of hepatocytes (PubMed : 34816459).
See full target information MYO5B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hepatology (Baltimore, Md.) 65:1655-1669 PubMed28027573

2017

Defects in myosin VB are associated with a spectrum of previously undiagnosed low γ-glutamyltransferase cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ling Qiu,Jing-Yu Gong,Jia-Yan Feng,Ren-Xue Wang,Jun Han,Teng Liu,Yi Lu,Li-Ting Li,Mei-Hong Zhang,Jonathan A Sheps,Neng-Li Wang,Yan-Yan Yan,Jia-Qi Li,Lian Chen,Christoph H Borchers,Bence Sipos,A S Knisely,Victor Ling,Qing-He Xing,Jian-She Wang
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Product promise

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