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AB230478

Anti-MYO6 antibody

5

(2 Reviews)

|

(5 Publications)

Rabbit Polyclonal MYO6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0389, MYO6, Unconventional myosin-VI, Unconventional myosin-6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (AB230478)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (AB230478)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (AB230478)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (AB230478)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MYO6 antibody (AB230478)
  • WB

Supplier Data

Western blot - Anti-MYO6 antibody (AB230478)

All lanes:

Western blot - Anti-MYO6 antibody (ab230478) at 1/500 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 149 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UM54

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYO6 also known as Myosin VI functions as a motor protein that moves towards the minus end of actin filaments. It has an approximate molecular mass of 140 kDa. MYO6 can express in various tissues including the inner ear and the cytoplasm of cells. Its unique ability to move in the opposite direction compared to other myosins makes it essential for processes like endocytosis and membrane trafficking.
Biological function summary

Myosin VI interacts with actin filaments to enable cellular processes like endocytosis autophagy and maintaining Golgi complex structure. MYO6 often part of a protein complex manages intracellular transportation and organelle positioning. By interacting with several binding partners it regulates the mechanical tension within cells contributing to cell shape and movement.

Pathways

The role of Myosin VI integrates with cell signaling and intracellular transport pathways. In the endocytic pathway it associates with proteins such as Dab2 and GIPC aiding in clathrin-mediated endocytosis. Myosin VI also participates within the autophagy pathway influencing cellular stress responses. These interactions demonstrate its involvement in the dynamic coordination of cellular components.

Abnormalities in MYO6 are linked to sensorineural hearing loss and forms of cancer. Mutations can lead to defects in cell maintenance and motility contributing to these conditions. In cancer Myosin VI may interact with proteins like E-cadherin affecting cell adhesion and metastasis. Understanding Myosin VI's connections to these diseases offers insights into possible therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosins are actin-based motor molecules with ATPase activity (By similarity). Unconventional myosins serve in intracellular movements (By similarity). Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments (PubMed : 10519557). Has slow rate of actin-activated ADP release due to weak ATP binding (By similarity). Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration (By similarity). Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway (PubMed : 16507995). Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells (PubMed : 11447109). Together with TOM1, mediates delivery of endocytic cargo to autophagosomes thereby promoting autophagosome maturation and driving fusion with lysosomes (PubMed : 23023224). Links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN (PubMed : 31371777). May act as a regulator of F-actin dynamics (By similarity). As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton (PubMed : 29467281). May play a role in transporting DAB2 from the plasma membrane to specific cellular targets (By similarity). May play a role in the extension and network organization of neurites (By similarity). Required for structural integrity of inner ear hair cells (By similarity). Required for the correct localization of CLIC5 and RDX at the stereocilium base (By similarity). Modulates RNA polymerase II-dependent transcription (PubMed : 16949370).
See full target information MYO6
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:21433 PubMed39271730

2024

Differential genotoxicity of Polygoni Multiflori in rat and human: insights from Ames test and S9 metabolic activation system.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Min Bak,Seng-Min Back,Da Yeon Kim,Soyoung Jung,Na-Young Jeung,Nan-Young Kim,Kang-Hyun Han,Yong-Bum Kim,Byoung-Seok Lee,Jun Hong Park,Hee Jun Cho,Hee Gu Lee,Ozkan Ozden,Sang Kyum Kim,Seong-Hoon Park

Nature communications 14:6190 PubMed37794006

2023

MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

He Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai Li

World journal of surgical oncology 19:299 PubMed34645476

2021

Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Ranran Yu,Chunhua Li,Yu Dang,Xiaoyu Yi,Lei Wang

World journal of surgical oncology 19:281 PubMed34535152

2021

LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingfeng Gu,Liang Dong,Yun Wang,Wenjia Nie,Wencong Liu,Ji-An Zhao

Cancer medicine 9:3115-3129 PubMed32130788

2020

Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyun Duan,Mei Xu,Meng Wu,Xiong Zhang,Min Gan,Hongbing Jiang
View all publications
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Product promise

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