Rabbit Polyclonal MYO6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- Nature communications 14:61902023MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Cao,Panpan Yang,Jia Liu,Yan Shao,Honghao Li,Pinglin Lai,Hong Wang,Anling Liu,Bin Guo,Yujin Tang,Xiaochun Bai,Kai LiPubMed 37794006
- World journal of surgical oncology 19:2992021Circ_0026416 downregulation blocks the development of colorectal cancer through depleting MYO6 expression by enriching miR-545-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhang,Ranran Yu,Chunhua Li,Yu Dang,Xiaoyu Yi,Lei WangPubMed 34645476
- World journal of surgical oncology 19:2812021LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingfeng Gu,Liang Dong,Yun Wang,Wenjia Nie,Wencong Liu,Ji-An ZhaoPubMed 34535152
- Cancer medicine 9:3115-31292020Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyun Duan et. al.PubMed 32130788
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM54
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Myosins are actin-based motor molecules with ATPase activity (By similarity). Unconventional myosins serve in intracellular movements (By similarity). Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments (PubMed:10519557). Has slow rate of actin-activated ADP release due to weak ATP binding (By similarity). Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration (By similarity). Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway (PubMed:16507995). Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells (PubMed:11447109). Together with TOM1, mediates delivery of endocytic cargo to autophagosomes thereby promoting autophagosome maturation and driving fusion with lysosomes (PubMed:23023224). Links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN (PubMed:31371777). May act as a regulator of F-actin dynamics (By similarity). As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton (PubMed:29467281). May play a role in transporting DAB2 from the plasma membrane to specific cellular targets (By similarity). May play a role in the extension and network organization of neurites (By similarity). Required for structural integrity of inner ear hair cells (By similarity). Required for the correct localization of CLIC5 and RDX at the stereocilium base (By similarity). Modulates RNA polymerase II-dependent transcription (PubMed:16949370).
Alternative names
KIAA0389, MYO6, Unconventional myosin-VI, Unconventional myosin-6
Recommended products
Rabbit Polyclonal MYO6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MYO6 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM54
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MYO6 also known as Myosin VI functions as a motor protein that moves towards the minus end of actin filaments. It has an approximate molecular mass of 140 kDa. MYO6 can express in various tissues including the inner ear and the cytoplasm of cells. Its unique ability to move in the opposite direction compared to other myosins makes it essential for processes like endocytosis and membrane trafficking.
- Biological function summary
Myosin VI interacts with actin filaments to enable cellular processes like endocytosis autophagy and maintaining Golgi complex structure. MYO6 often part of a protein complex manages intracellular transportation and organelle positioning. By interacting with several binding partners it regulates the mechanical tension within cells contributing to cell shape and movement.
- Pathways
The role of Myosin VI integrates with cell signaling and intracellular transport pathways. In the endocytic pathway it associates with proteins such as Dab2 and GIPC aiding in clathrin-mediated endocytosis. Myosin VI also participates within the autophagy pathway influencing cellular stress responses. These interactions demonstrate its involvement in the dynamic coordination of cellular components.
- Associated diseases and disorders
Abnormalities in MYO6 are linked to sensorineural hearing loss and forms of cancer. Mutations can lead to defects in cell maintenance and motility contributing to these conditions. In cancer Myosin VI may interact with proteins like E-cadherin affecting cell adhesion and metastasis. Understanding Myosin VI's connections to these diseases offers insights into possible therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-MYO6 antibody (ab230478)
All lanes: Western blot - Anti-MYO6 antibody (ab230478) at 1/500 dilution
All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 149 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (ab230478)
Formalin-fixed, paraffin-embedded human kidney tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO6 antibody (ab230478)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for MYO6 using ab230478 at 1/100 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com