AB41552

Anti-Myocilin antibody

Name: Anti-Myocilin antibody. Rabbit polyclonal (ab41552) | Abcam
Brand: Abcam Limited
SKU: AB41552
Price: 410 USD
Availability: InStock

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(20 Publications)

Rabbit Polyclonal Myocilin antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Monkey, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human MYOC aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GLC1A, TIGR, MYOC, Myocilin, Myocilin 55 kDa subunit, Trabecular meshwork-induced glucocorticoid response protein

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myocilin antibody (AB41552)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myocilin antibody (AB41552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labelling Myocilin with ab41552 at a dilution of 1/50. Counterstained with hematoxylin (blue). 40X magnigication.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Monkey, Human, Rat

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human MYOC aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q99972

Specificity

The antibody does not cross react with any other protein in RPE cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myocilin also known as Trabecular Meshwork Inducible Glucocorticoid Response protein (TIGR) is a protein mainly involved in ocular processes. It weighs about 55 kDa and contains several key domains including a leucine zipper motif and an olfactomedin-like domain. Myocilin is abundantly expressed in the trabecular meshwork of the eye skeletal muscle heart and brain. The protein appears in both intracellular and secreted forms and undergoes glycosylation.

Biological function summary

This protein plays an important role in the regulation of intraocular pressure by influencing the outflow of aqueous humor through the trabecular meshwork. It is also known to be part of a protein complex that interacts with other extracellular matrix components to maintain the structural integrity of tissues. Beyond the eye Myocilin similarly contributes to cellular processes in other tissues assisting in maintaining cellular structure and integrity.

Pathways

Myocilin functions within the Wnt signaling pathway and extracellular matrix-receptor interaction pathway. These pathways are significant in regulating cell growth adhesion and differentiation in various tissues. In the Wnt signaling pathway Myocilin interacts with proteins such as Frizzled receptors affecting the downstream effects on cell behavior and fate. In the extracellular matrix pathway Myocilin associates with collagens and laminins influencing tissue architecture and dynamics.

Myocilin plays a significant role in glaucoma and Myocilin-related ocular hypertension. Glaucoma often involves elevated intraocular pressure due to impaired aqueous humor outflow in which mutated Myocilin may not function properly affecting cellular interactions and contributing to disease progression. Links have been identified with proteins like optineurin in the context of glaucoma where these proteins can collectively influence disease pathophysiology and lead to optic nerve damage if dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.

See full target information MYOC

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2925:259-270 PubMed40498196

2025

Preparation of Human Trabecular Meshwork Cells for Metabolomic Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie How,Gelila B Yohannes,Katelyn Kane,Isabella G Moceri,Becca N Lees,Joshua J Ostler,Sanjoy K Bhattacharya,Anh H Pham

Investigative ophthalmology & visual science 66:25 PubMed40067295

2025

Silicone Oil Affects Fibrosis of Human Trabecular Meshwork Cells by Upregulating Ferroptosis Through a ROS/NOX4/Smad3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Yang Zhang,Huimin Zhong,Yumeng Zhang,Ruiqi Han,Yanzhi Guo,Shouyue Huang,Huan Yu,Yisheng Zhong

Scientific reports 15:5049 PubMed39934637

2025

A novel lncRNA enhances autophagy to suppress extracellular matrix via modulating TP53INP1 in human trabecular meshwork cells under oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjun Tie,Junhong Guo,Yijia Huang,Zihan Huang,Zhichao Yan,Jiemei Yuan,Xiaoli Shen,Jiantao Wang

PNAS nexus 4:pgae556 PubMed39726989

2024

Antibody-mediated clearance of an ER-resident aggregate that causes glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Minh Thu Ma,Ahlam N Qerqez,Kamisha R Hill,Laura R Azouz,Hannah A Youngblood,Shannon E Hill,Yemo Ku,Donna M Peters,Jennifer A Maynard,Raquel L Lieberman

Scientific reports 14:6958 PubMed38521856

2024

Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti V Patil,Balasankara Reddy Kaipa,Sujata Ranshing,Yogapriya Sundaresan,J Cameron Millar,Bhavani Nagarajan,Charles Kiehlbauch,Qihong Zhang,Ankur Jain,Charles C Searby,Todd E Scheetz,Abbot F Clark,Val C Sheffield,Gulab S Zode

International journal of molecular sciences 24: PubMed37511406

2023

Sigma-1 Receptor Agonist Fluvoxamine Ameliorates Fibrotic Response of Trabecular Meshwork Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Hodrea,Minh Ngoc Tran,Balazs Besztercei,Timea Medveczki,Attila J Szabo,Laszlo Őrfi,Illes Kovacs,Andrea Fekete

Journal of nanobiotechnology 20:459 PubMed36303134

2022

Towards preventing exfoliation glaucoma by targeting and removing fibrillar aggregates associated with exfoliation syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Ghaffari Sharaf,Kosala D Waduthanthri,Andrew Crichton,Karim F Damji,Larry D Unsworth

Current eye research 47:1165-1178 PubMed35481448

2022

TGFβ2 Regulates Human Trabecular Meshwork Cell Contractility ERK and ROCK Pathways with Distinct Signaling Crosstalk Dependent on the Culture Substrate.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Li,Jessica L Henty-Ridilla,Audrey M Bernstein,Preethi S Ganapathy,Samuel Herberg

International journal of nanomedicine 17:1285-1307 PubMed35345785

2022

Magnetic Nano-Platform Enhanced iPSC-Derived Trabecular Meshwork Delivery and Tracking Efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangji Wang,Qilong Cao,Shen Wu,Mohammad Reza Bahrani Fard,Ningli Wang,Jie Cao,Wei Zhu

International journal of ophthalmology 15:388-393 PubMed35310043

2022

Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Koichi Yoshida,Motofumi Kawai,Tsugiaki Utsunomiya,Akihiro Ishibazawa,Young-Seok Song,Mariana Sayuri B Udo,Yoshikazu Tasaki,Akitoshi Yoshida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myocilin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myocilin antibody (AB41552)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise