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Rabbit Recombinant Monoclonal Myocilin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] - BSA and Azide free (AB318198), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] - BSA and Azide free (AB318198), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] - BSA and Azide free (AB318198), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] - BSA and Azide free (AB318198), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] - BSA and Azide free (AB318198), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IPIHC-P
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.

Alternative names

GLC1A, TIGR, MYOC, Myocilin, Myocilin 55 kDa subunit, Trabecular meshwork-induced glucocorticoid response protein

Recommended products

Rabbit Recombinant Monoclonal Myocilin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28799-15
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab318198 is the carrier-free version of Anti-Myocilin antibody [EPR28799-15] ab318197.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Myocilin also known as Trabecular Meshwork Inducible Glucocorticoid Response protein (TIGR) is a protein mainly involved in ocular processes. It weighs about 55 kDa and contains several key domains including a leucine zipper motif and an olfactomedin-like domain. Myocilin is abundantly expressed in the trabecular meshwork of the eye skeletal muscle heart and brain. The protein appears in both intracellular and secreted forms and undergoes glycosylation.

Biological function summary

This protein plays an important role in the regulation of intraocular pressure by influencing the outflow of aqueous humor through the trabecular meshwork. It is also known to be part of a protein complex that interacts with other extracellular matrix components to maintain the structural integrity of tissues. Beyond the eye Myocilin similarly contributes to cellular processes in other tissues assisting in maintaining cellular structure and integrity.

Pathways

Myocilin functions within the Wnt signaling pathway and extracellular matrix-receptor interaction pathway. These pathways are significant in regulating cell growth adhesion and differentiation in various tissues. In the Wnt signaling pathway Myocilin interacts with proteins such as Frizzled receptors affecting the downstream effects on cell behavior and fate. In the extracellular matrix pathway Myocilin associates with collagens and laminins influencing tissue architecture and dynamics.

Associated diseases and disorders

Myocilin plays a significant role in glaucoma and Myocilin-related ocular hypertension. Glaucoma often involves elevated intraocular pressure due to impaired aqueous humor outflow in which mutated Myocilin may not function properly affecting cellular interactions and contributing to disease progression. Links have been identified with proteins like optineurin in the context of glaucoma where these proteins can collectively influence disease pathophysiology and lead to optic nerve damage if dysregulated.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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