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AB203383

Anti-MyoD1 antibody

3

(3 Reviews)

|

(23 Publications)

Rabbit Polyclonal MYOD1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Mouse Myod1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHC1, MYF3, MYOD, MYOD1, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Myf-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody (AB203383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody (AB203383)

Immunohistochemical analysis of formalin-fixed and paraffin embedded embryonic rhabdomyoma tissue labeling MyoD1using ab203333 at 1/200, followed by secondary detection and DAB staining.

Western blot - Anti-MyoD1 antibody (AB203383)
  • WB

Supplier Data

Western blot - Anti-MyoD1 antibody (AB203383)

All lanes:

Western blot - Anti-MyoD1 antibody (ab203383) at 1/300 dilution

Lane 1:

Rat muscle lysate

Lane 2:

Mouse muscle lysate

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Myod1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10085

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.
Biological function summary

MyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.

Pathways

MyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.

MyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
See full target information MYOD1
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Journal of cosmetic dermatology 24:e16749 PubMed39719683

2024

Focused Electric Field Technology: A Novel Myoelectrical Stimulation Technology for Noninvasive Aging Muscle Rejuvenation.

Applications

Unspecified application

Species

Unspecified reactive species

Boyang Jiang,Bingbing Chen,Jia Xu,Jieshu Luo,Min Yao

BMB reports 57:539-545 PubMed39567207

2024

Recombinant ADAMTS1 promotes muscle cell differentiation and alleviates muscle atrophy by repressing NOTCH1.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Hyup Lee,Sang Yoon Kim,Yun Gu Gwon,Chanwoo Lee,Changhwan Kim,Ick Hyun Cho,Tae-Won Kim,Bong-Keun Choi

Orthopaedic journal of sports medicine 12:23259671241252421 PubMed38840789

2024

Influence of Frequent Corticosteroid Local Injections on the Expression of Genes and Proteins Related to Fatty Infiltration, Muscle Atrophy, Inflammation, and Fibrosis in Patients With Chronic Rotator Cuff Tears: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

JiHwan Lee,Taewoo Lho,Jongwon Lee,Jeongkun Lee,Seok Won Chung

NPJ microgravity 10:18 PubMed38365862

2024

Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanping Ren,Eun Hyun Ahn,Minjae Do,Devin B Mair,Amir Monemianesfahani,Peter H U Lee,Deok-Ho Kim

Intractable & rare diseases research 12:180-190 PubMed37662621

2023

The novel role of IFITM1-3 in myogenic differentiation of C2C12 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtao Zhang,Yanqin Lu,Xianxian Li,Shanshan Zhang,Pengchao Liu,Xiaoyang Hao,Jinxiang Han

Advanced healthcare materials 12:e2203056 PubMed36782053

2023

Hierarchically Injectable Hydrogel Sequentially Delivers AntagomiR-467a-3p-Loaded and AntagomiR-874-5p-Loaded Satellite-Cell-Targeting Bioengineered Extracellular Vesicles Attenuating Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Hanhao Dai,Jun Luo,Lili Deng,Chao Song,Zhibo Deng,Yijing Wu,Song Gu,Jie Xu

Evidence-based complementary and alternative medicine : eCAM 2022:2695679 PubMed35966754

2022

Electroacupuncture of Weizhong (BL-40) Acupoint Inspires Muscular Satellite Cell Regeneration and Promotes Muscle Repair Capacity after Back Muscle Injury in Sprague-Dawley Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-Xiu Huo,Zhi-Ling Wang,Ying-Qian Jiao,Xiao-Yi Wang,Yan-Li Lang,Yong-Jun Mi,Zhi-Xin Li,Zhi-Zhong Ma

Journal of orthopaedic translation 35:23-36 PubMed35846725

2022

Inhibiting uptake of extracellular vesicles derived from senescent bone marrow mesenchymal stem cells by muscle satellite cells attenuates sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Hanhao Dai,Wu Zheng,Jun Luo,Guoyu Yu,Chao Song,Yijing Wu,Jie Xu

Frontiers in endocrinology 12:693977 PubMed34484115

2021

An Study on Extracellular Vesicles From Adipose-Derived Mesenchymal Stem Cells in Protecting Stress Urinary Incontinence Through MicroRNA-93/F3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Yali Wang,Yuancui Xiang,Jinping Ma,Hui Zhang,Jingfang Dai,Yanan Hou,Yupei Yang,Jingru Ma,Hongjuan Li

International journal of molecular medicine 48: PubMed34080654

2021

Role and mechanism underlying FoxO6 in skeletal muscle and .

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Yiyi Zhang,Min Zhou,Shuang Wang,Tiane Li,Zhangyong Hu,Chengwu Jin
View all publications
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