AB209976

Anti-MyoD1 antibody

Lab Essentials
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(3 Publications)

Rabbit Polyclonal MYOD1 antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish myod1 aa 100 to C-terminus.

View Alternative Names

myod, myod1, Myoblast determination protein 1 homolog, Myogenic factor 1

1 Images

IHC - Wholemount - Anti-MyoD1 antibody (AB209976)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-MyoD1 antibody (AB209976)

Immunohistochemical analysis of Paraformaldehyde-fixed whole mount Zebrafish embryos labeling MyoD1 at Somites with ab209976 at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Zebrafish myod1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q90477

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.

Biological function summary

MyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.

Pathways

MyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.

MyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

May act as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation.

See full target information myod1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:5635-5666 PubMed39349846

2024

Carboxy-terminal polyglutamylation regulates signaling and phase separation of the Dishevelled protein.

Applications

Unspecified application

Species

Unspecified reactive species

Marek Kravec,Ondrej Šedo,Jana Nedvědová,Miroslav Micka,Marie Šulcová,Nikodém Zezula,Kristína Gömöryová,David Potěšil,Ranjani Sri Ganji,Sara Bologna,Igor Červenka,Zbyněk Zdráhal,Jakub Harnoš,Konstantinos Tripsianes,Carsten Janke,Cyril Bařinka,Vítězslav Bryja

RSC advances 13:14685-14697 PubMed37197682

2023

Integrated pipeline for ultrasensitive protein detection in cancer nanomedicine.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-An Cheng,Li-Chiao Chiang,Yu-Syuan Chu

NPJ science of food 7:17 PubMed37149658

2023

Tissue-like cultured fish fillets through a synthetic food pipeline.

Applications

Unspecified application

Species

Unspecified reactive species

Enbo Xu,Ruihao Niu,Jihui Lao,Shengliang Zhang,Jie Li,Yiyuan Zhu,Huimin Shi,Qingqing Zhu,Yijian Chen,Yuyan Jiang,Wenjun Wang,Jun Yin,Qihe Chen,Xiao Huang,Jun Chen,Donghong Liu

View all publications

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