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Rabbit Monoclonal MYOD1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Recombinant fragment - Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377), expandable thumbnail
  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377), expandable thumbnail
  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Expected
Tested
Not recommended
Mouse
Tested
Tested
Not recommended
Recombinant fragment - Mouse
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Recombinant fragment - Mouse
Dilution info
-
Notes

-

Target data

Function

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed:16901893). Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (PubMed:21798092, PubMed:3175662).

Alternative names

Myod, Myod1, Myoblast determination protein 1

Rabbit Monoclonal MYOD1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Recombinant fragment - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29627-738
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse spleen. The section was incubated with ab323377 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse cardiac muscle. The section was incubated with ab323377 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Immunohistochemical analysis of paraffin-embedded Mouse E12 embryo tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on skeletal muscle of mouse E12 embryo. The section was incubated with ab323377 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa (PMID: 17028574 )

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

    Lane 1: Mouse E14 forelimb tissue lysate at 40 µg

    Lane 2: Mouse E14 hindlimb tissue lysate at 40 µg

    Lane 3: Mouse embryo tissue lysate at 40 µg

    Lane 4: RD(human muscle mtinucleated spindle-shaped cell) whole lysate at 40 µg

    Lane 5: HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 47 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with mouse MYF5, MYF6 or MYOG.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

    Lane 1: His-tagged mouse MYOD1 recombinant protein at 10 ng

    Lane 2: His-tagged mouse MYF5 recombinant protein at 10 ng

    Lane 3: His-tagged mouse MYF6 recombinant protein at 10 ng

    Lane 4: His-tagged mouse MYOG recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 40 kDa

    Exposure time: 37s

  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: brain, heart, kidney, spleen(HPA data)

    This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

    Lane 1: Mouse E12 embryo tissue lysate at 20 µg

    Lane 2: Mouse E14 embryo tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 47 kDa, 36 kDa

    Exposure time: 125s

  • Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377), expandable thumbnail

    Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression of MyoD1 is downregulated in response to C2C12 differentiation for 5 days treatment(DOI: 10.4236/cellbio.2013.23016)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

    Lane 1: Untreated C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 2: C2C12 differentiation to muscle for 5 days whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 47 kDa, 36 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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