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AB240073

Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free

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Rabbit Recombinant Monoclonal MYOD1 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, ICC/IF, IHC-P, WB and reacts with Human samples.

View Alternative Names

BHLHC1, MYF3, MYOD, MYOD1, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Myf-3

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ChIC/CUT&RUN sequencing - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 RD (Human muscle rhabdomyosarcoma) cells and 5µg of ab133627 [EPR6653-131]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

ab133627 showing negative staining in Normal kidney tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

Immunohistochemical analysis of MyoD1 in paraffin embedded Human rhabdomyosarcoma tissue, using ab133627 at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

ab133627 showing negative staining in Normal heart tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

ab133627 showing negative staining in Thyroid gland carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

ab133627 showing negative staining in Skeletal muscle tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133627).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6653-131

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ChIC/CUT&RUN-seq, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Abcam recommends using milk as the blocking agent.</p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.
Biological function summary

MyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.

Pathways

MyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.

MyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
See full target information MYOD1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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chicCutRunSequencingBooklet
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