AB76746

Anti-Myoferlin antibody [7D2]

Name: Anti-Myoferlin antibody [7D2] (ab76746) | Abcam
Brand: Abcam Limited
SKU: AB76746
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(8 Publications)

Mouse Monoclonal Myoferlin antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MYOF.

View Alternative Names

FER1L3, KIAA1207, MYOF, Myoferlin, Fer-1-like protein 3

2 Images

Flow Cytometry - Anti-Myoferlin antibody [7D2] (AB76746)

Flow Cyt

Unknown

Flow Cytometry - Anti-Myoferlin antibody [7D2] (AB76746)

Overlay histogram showing HeLa cells stained with ab76746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76746, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (AB76746)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (AB76746)

ICC/IF image of ab76746 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7D2

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human MYOF. The exact immunogen used to generate this antibody is proprietary information.

Q9NZM1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myoferlin alternatively known as MYOF is a member of the ferlin family proteins and plays a mechanical role in membrane repair and fusion. It has a molecular mass of approximately 230 kDa. Myoferlin expresses mainly in muscle and endothelial tissues. Its function is critical during muscle development and regeneration as it participates in the fusion of myoblasts into mature myotubes. Also Myoferlin is involved in the repair of damaged sarcolemmal membranes in muscle cells.

Biological function summary

The protein supports membrane dynamics and stabilization. Myoferlin is not part of a large complex but interacts with other proteins at the cellular membrane. It regulates cellular processes such as proliferation differentiation and endocytosis. In muscle cells it assists in the organization and function of the cytoskeleton maintaining proper cellular architecture and signaling.

Pathways

Myoferlin engages in critical roles in pathways related to cell membrane repair and trafficking. It associates with pathways like the myogenesis pathway and integrin signaling. In these pathways it interacts with other proteins such as dysferlin and caveolin-3 which facilitate membrane repair and receptor localization respectively. Myoferlin ensures efficient cellular response to membrane damage maintaining tissue integrity.

Myoferlin is connected to muscular dystrophies and cancer. It correlates with the progression of muscular disorder due to its deficiency in membrane repair mechanisms. Additionally in cancer its expression levels relate to tumor invasion and metastasis involving interactions with proteins like E-cadherin and MMP-9. Investigating Myoferlin's role can aid in understanding its potential as a therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).

See full target information MYOF

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of pathology and translational medicine 56:199-204 PubMed35535365

2022

Correlation between myoferlin expression and lymph node metastasis in papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Min Na,Dong Chul Kim,Dae Hyun Song,Hyo Jung An,Hyun Min Koh,Jeong-Hee Lee,Jong Sil Lee,Jung Wook Yang,Min Hye Kim

Scientific reports 9:12656 PubMed31477752

2019

Myoferlin silencing inhibits VEGFR2-mediated proliferation of metastatic clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo Jung An,Dae Hyun Song,Hyun Min Koh,Yu-Min Kim,Gyung Hyuck Ko,Jeong-Hee Lee,Jong Sil Lee,Jung Wook Yang,Min Hye Kim,Deok Ha Seo,Se Min Jang,Dong Chul Kim

Frontiers in genetics 10:608 PubMed31297131

2019

Truncating Variant in Gene Is Associated With Limb-Girdle Type Muscular Dystrophy and Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Artem Kiselev,Raquel Vaz,Anastasia Knyazeva,Alexey Sergushichev,Renata Dmitrieva,Aleksandr Khudiakov,John Jorholt,Natalia Smolina,Ksenia Sukhareva,Yulia Fomicheva,Evgeny Mikhaylov,Lubov Mitrofanova,Alexander Predeus,Gunnar Sjoberg,Dmitriy Rudenko,Thomas Sejersen,Anna Lindstrand,Anna Kostareva

Oncotarget 8:89033-89039 PubMed29179496

2017

Prognostic role of myoferlin expression in patients with clear cell renal cell carcinoma.

Applications

IHC-P

Species

Human

Dae Hyun Song,Gyung Hyuck Ko,Jeong Hee Lee,Jong Sil Lee,Jung Wook Yang,Min Hye Kim,Hyo Jung An,Myoung Hee Kang,Kyung Nyeo Jeon,Dong Chul Kim

Cellular signalling 33:30-40 PubMed28192161

2017

Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Ann-Katrin Piper,Samuel E Ross,Gregory M Redpath,Frances A Lemckert,Natalie Woolger,Adam Bournazos,Peter A Greer,Roger B Sutton,Sandra T Cooper

Oncology letters 11:998-1006 PubMed26893682

2016

Myoferlin expression in non-small cell lung cancer: Prognostic role and correlation with VEGFR-2 expression.

Applications

IHC

Species

Unspecified reactive species

Dae Hyun Song,Gyung Hyuck Ko,Jeong Hee Lee,Jong Sil Lee,Gyeong-Won Lee,Hyeon Cheol Kim,Jung Wook Yang,Rok Won Heo,Gu Seob Roh,Sun-Young Han,Dong Chul Kim

Stem cells (Dayton, Ohio) 32:2767-79 PubMed24905975

2014

sdf1 Expression reveals a source of perivascular-derived mesenchymal stem cells in zebrafish.

Applications

Flow Cyt

Species

Zebrafish

Troy C Lund,Xiaobai Patrinostro,Ashley C Kramer,Paul Stadem,Lee Ann Higgins,Todd W Markowski,Matt S Wroblewski,Diane S Lidke,Jakub Tolar,Bruce R Blazar

PloS one 7:e40478 PubMed22808170

2012

Expression of myoferlin in human airway epithelium and its role in cell adhesion and zonula occludens-1 expression.

Applications

ICC/IF, WB, IHC-P

Species

Human, Human, Human

Cleo Leung,Furquan Shaheen,Pascal Bernatchez,Tillie-Louise Hackett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myoferlin antibody [7D2]

Flow Cytometry - Anti-Myoferlin antibody [7D2] (AB76746)

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [7D2] (AB76746)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise