AB224091

Anti-Myoferlin antibody

Name: Anti-Myoferlin antibody (ab224091) | Abcam
Brand: Abcam Limited
SKU: AB224091
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Myoferlin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYOF aa 100-250.

View Alternative Names

FER1L3, KIAA1207, MYOF, Myoferlin, Fer-1-like protein 3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Paraffin-embedded human prostate tissue stained for Myoferlin using ab24091 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody (AB224091)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody (AB224091)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Myoferlin (green) using ab224091 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Paraffin-embedded human bladder tissue stained for Myoferlin using ab24091 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Paraffin-embedded human placenta tissue stained for Myoferlin using ab24091 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Myoferlin antibody (AB224091)

All lanes:

Western blot - Anti-Myoferlin antibody (ab224091) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 235 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MYOF aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NZM1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myoferlin alternatively known as MYOF is a member of the ferlin family proteins and plays a mechanical role in membrane repair and fusion. It has a molecular mass of approximately 230 kDa. Myoferlin expresses mainly in muscle and endothelial tissues. Its function is critical during muscle development and regeneration as it participates in the fusion of myoblasts into mature myotubes. Also Myoferlin is involved in the repair of damaged sarcolemmal membranes in muscle cells.

Biological function summary

The protein supports membrane dynamics and stabilization. Myoferlin is not part of a large complex but interacts with other proteins at the cellular membrane. It regulates cellular processes such as proliferation differentiation and endocytosis. In muscle cells it assists in the organization and function of the cytoskeleton maintaining proper cellular architecture and signaling.

Pathways

Myoferlin engages in critical roles in pathways related to cell membrane repair and trafficking. It associates with pathways like the myogenesis pathway and integrin signaling. In these pathways it interacts with other proteins such as dysferlin and caveolin-3 which facilitate membrane repair and receptor localization respectively. Myoferlin ensures efficient cellular response to membrane damage maintaining tissue integrity.

Myoferlin is connected to muscular dystrophies and cancer. It correlates with the progression of muscular disorder due to its deficiency in membrane repair mechanisms. Additionally in cancer its expression levels relate to tumor invasion and metastasis involving interactions with proteins like E-cadherin and MMP-9. Investigating Myoferlin's role can aid in understanding its potential as a therapeutic target in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).

See full target information MYOF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu5091 PubMed40203102

2025

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Felix Kraus,João P L Coelho,Muskaan Vasandani,Jiuchun Zhang,Benjamin M Adams,Joao A Paulo,J Wade Harper,Sichen Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myoferlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody (AB224091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoferlin antibody (AB224091)

Western blot - Anti-Myoferlin antibody (AB224091)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise