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AB220961

Anti-MyoGEF antibody

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(1 Publication)

Rabbit Polyclonal MyoGEF antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PLEKHG6 aa 1-100.

View Alternative Names

Pleckstrin homology domain-containing family G member 6, PH domain-containing family G member 6, Myosin-interacting guanine nucleotide exchange factor, MyoGEF, PLEKHG6

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-MyoGEF antibody (AB220961)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MyoGEF antibody (AB220961)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized CACO-2 cell line labeling MyoGEF with ab220961 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PLEKHG6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q3KR16

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MyoGEF also known as Myosin II interacting GEF mechanistically acts as a guanine nucleotide exchange factor that regulates the activation of RhoA by facilitating the exchange of GDP for GTP. MyoGEF weighing approximately 85 kDa is expressed in various tissues with notable presence in muscle and neuronal cells. It specifically localizes to the mitotic spindle and cleavage furrow during cell division indicating its role in cytokinesis.
Biological function summary

MyoGEF participates in controlling the cytoskeleton through its involvement in actin-related processes. These actions are important during cell migration and mitosis where it assists formation and contraction of the actomyosin ring. MyoGEF works in conjunction with other molecules like Myosin II as part of larger protein complexes to manage cellular shape and transport functionalities.

Pathways

MyoGEF functions prominently within the RhoA signaling and cell division pathways. In the RhoA signaling pathway it interacts with Rho kinases and modulates activities involving cytoskeletal dynamics. Through the cell division pathway MyoGEF ensures correct cytokinesis and is linked to proteins such as Ect2 which is essential for proper cell cycle progression.

There is involvement of MyoGEF in cancer progression and neurological disorders. Its dysregulation can be tied to improper cell division contributing to tumorigenesis. Additionally MyoGEF's connection to cytoskeletal abnormalities links it to neurological disorders where interactions with proteins like ROCK1 can lead to disrupted neuronal signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.
See full target information PLEKHG6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou
View all publications
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