AB238027

Anti-Myogenin antibody [MYOG/2660]

Name: Anti-Myogenin antibody [MYOG/2660] (ab238027) | Abcam
Brand: Abcam Limited
SKU: AB238027

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(1 Publication)

Mouse Monoclonal Myogenin antibody. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYOG.

View Alternative Names

BHLHC3, MYF4, MYOG, Myogenin, Class C basic helix-loop-helix protein 3, Myogenic factor 4, bHLHc3, Myf-4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [MYOG/2660] (AB238027)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [MYOG/2660] (AB238027)

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma tissue stained for Myogenin using ab238027 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Myogenin antibody [MYOG/2660] (AB238027)

Protein Array

Unknown

Protein Array - Anti-Myogenin antibody [MYOG/2660] (AB238027)

ab238027 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MYOG/2660

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MYOG.

P15173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myogenin also known as MYOG is a muscle-specific transcription factor that plays an important role in the regulation of muscle differentiation and development. It belongs to the MyoD family of basic helix-loop-helix proteins. Myogenin has a molecular weight of approximately 34 to 39 kDa. It is typically expressed in skeletal muscle tissues aiding in the transition from proliferating myoblasts to differentiated myotubes. Immunohistochemistry (IHC) methods often use myogenin antibodies like the 'myogenin anticuerpo' and 'F5D' to identify myogenin expression within tissue samples facilitating the understanding of muscle tissue organization and development.

Biological function summary

Myogenin functions in controlling the transcription of muscle-specific genes that are essential during myogenesis. This transcription factor forms heterodimers with E-proteins becoming part of DNA-binding complexes that activate the transcription of muscle-specific genes. Myogenin's presence is critical during muscle cell fusion and maturation signifying its importance in proper muscle tissue formation and maintenance. Myogenin staining is often used as a technique to study muscle differentiation in various experimental settings.

Pathways

Myogenin is integrated into the myogenic regulatory network where it activates genes involved in muscle fiber maturation. It participates in the MyoD regulatory pathway working alongside other myogenic regulatory factors like MyoD Myf5 and MRF4. These proteins create a coordinated network ensuring efficient muscle cell differentiation and growth. The cross-talk between myogenin and these factors highlights its role in the intricate web of gene regulation necessary for muscle development.

The altered expression or mutation of the myogenin gene has potential implications in muscular dystrophies and myopathies. For instance disruptions in myogenin activity can lead to impaired muscle repair and regeneration contributing to the pathology of conditions such as rhabdomyosarcoma. Within these contexts myogenin associates with other proteins like MyoD whose expression might also be altered further accentuating the disruption in normal muscle differentiation processes during disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Also cooperates with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Also plays a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).

See full target information MYOG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal, genetic engineering & biotechnology 23:100496 PubMed40390503

2025

Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sudipta Deb Nath,Md Tamzid Hossain Tanim,Md Mahmudul Hasan Akash,Mohammad Golam Mostafa,Abu Ashfaqur Sajib

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Myogenin antibody [MYOG/2660]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [MYOG/2660] (AB238027)

Protein Array - Anti-Myogenin antibody [MYOG/2660] (AB238027)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise