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AB47702

Anti-Myoglobin antibody [6H8B5]

4

(1 Review)

|

(2 Publications)

Mouse Monoclonal Myoglobin antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MB.

View Alternative Names

Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, MB

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [6H8B5] (AB47702)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [6H8B5] (AB47702)

ab47702 staining human Myoglobin in human skeletal muscle tissue, showing cytoplasmic localization, with DAB staining.

Western blot - Anti-Myoglobin antibody [6H8B5] (AB47702)
  • WB

Unknown

Western blot - Anti-Myoglobin antibody [6H8B5] (AB47702)

All lanes:

Western blot - Anti-Myoglobin antibody [6H8B5] (ab47702)

All lanes:

Truncated Myoglobin recombinant protein (2-154aa) (Tagged)

Predicted band size: 17 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6H8B5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MB.

P02144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR232969-1 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.
Biological function summary

Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.

Pathways

The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.

Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed : 30918256, PubMed : 34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed : 32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed : 34679218).
See full target information MB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of veterinary medical science 85:344-357 PubMed36709969

2023

Reconstruction of a partial esophageal defect using tunica vaginalis and buccal mucosa autograft: an experimental study in mongrel dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Hashem,Elsayed Metwally,Yasmina K Mahmoud,Ibrahim E Helal,Mahmoud F Ahmed

Metabolism: clinical and experimental 59:1000-11 PubMed20045141

2010

Methionine restriction effects on mitochondrial biogenesis and aerobic capacity in white adipose tissue, liver, and skeletal muscle of F344 rats.

Applications

WB

Species

Rat

Carmen E Perrone,Dwight A L Mattocks,Maureen Jarvis-Morar,Jason D Plummer,Norman Orentreich
View all publications
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