Rabbit Polyclonal Myoglobin antibody. Suitable for WB, sELISA and reacts with Human samples.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
WB | sELISA | |
---|---|---|
Human | Tested | Tested |
Chimpanzee | Predicted | Predicted |
Gorilla | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [BDI927] to Myoglobin (ab19607) as Capture. |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
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Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:30918256, PubMed:34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed:32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed:34679218).
Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, MB
Rabbit Polyclonal Myoglobin antibody. Suitable for WB, sELISA and reacts with Human samples.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Myoglobin antibody (ab78441) at 0.5 µg/mL
Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 5 µg
Lane 2: Human heart tissue lysate - total protein (ab29431) at 5 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 10s
All lanes: Western blot - Anti-Myoglobin antibody (ab78441) at 0.5 µg/mL
Lane 1: Western blot - Native Human Myoglobin protein (Native Human Myoglobin protein ab77876) at 0.1 µg
Lane 2: Western blot - Native Human Myoglobin protein (Native Human Myoglobin protein ab77876) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Exposure time: 2min
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