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Rabbit Polyclonal Myoglobin antibody. Suitable for WB, sELISA and reacts with Human samples.

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Images

ELISA - Anti-Myoglobin antibody (AB78441), expandable thumbnail
  • Western blot - Anti-Myoglobin antibody (AB78441), expandable thumbnail
  • Western blot - Anti-Myoglobin antibody (AB78441), expandable thumbnail
  • Sandwich ELISA - Anti-Myoglobin antibody (AB78441), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Human
Tested
Tested
Chimpanzee
Predicted
Predicted
Gorilla
Predicted
Predicted
Orangutan
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

0.5 µg/mL

Notes

-

Predicted
Predicted

Species

Chimpanzee, Gorilla, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

For sandwich ELISA, use this antibody as Detection at 0.5 μg/ml with Mouse monoclonal [BDI927] to Myoglobin (ab19607) as Capture.

Predicted
Predicted

Species

Chimpanzee, Gorilla, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:30918256, PubMed:34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed:32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed:34679218).

Alternative names

Recommended products

Rabbit Polyclonal Myoglobin antibody. Suitable for WB, sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • ELISA - Anti-Myoglobin antibody (ab78441), expandable thumbnail

    ELISA - Anti-Myoglobin antibody (ab78441)

    Standard Curve for Myoglobin (Analyte: Myoglobin protein (ab96036)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI927] to Myoglobin (ab19607) at 5µg/ml and Detector Antibody Rabbit polyclonal to Myoglobin (ab78441) at 0.5µg/ml.

  • Western blot - Anti-Myoglobin antibody (ab78441), expandable thumbnail

    Western blot - Anti-Myoglobin antibody (ab78441)

    All lanes: Western blot - Anti-Myoglobin antibody (ab78441) at 0.5 µg/mL

    Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 5 µg

    Lane 2: Human heart tissue lysate - total protein (ab29431) at 5 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

    Exposure time: 10s

  • Western blot - Anti-Myoglobin antibody (ab78441), expandable thumbnail

    Western blot - Anti-Myoglobin antibody (ab78441)

    All lanes: Western blot - Anti-Myoglobin antibody (ab78441) at 0.5 µg/mL

    Lane 1: Western blot - Native Human Myoglobin protein (Native Human Myoglobin protein ab77876) at 0.1 µg

    Lane 2: Western blot - Native Human Myoglobin protein (Native Human Myoglobin protein ab77876) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa

    Exposure time: 2min

  • Sandwich ELISA - Anti-Myoglobin antibody (ab78441), expandable thumbnail

    Sandwich ELISA - Anti-Myoglobin antibody (ab78441)

    Standard Curve for Myoglobin (Analyte: Myoglobin protein (ab96036)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [BDI927] to Myoglobin (ab19607) at 5μg/ml and Detector Antibody Rabbit polyclonal to Myoglobin (ab78441) at 0.5μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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