Name: Anti-Myoglobin antibody [EP3081Y]
SKU: ab77232
Rating: 5 (1 reviews)

Anti-Myoglobin antibody [EP3081Y] (ab77232) is a rabbit monoclonal antibody that is used to detect Myoglobin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 30 publications

-Over 30 publications

Related conjugates and formulations

ab232388
Carrier free
Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free
ab199243
Conjugated
HRP Anti-Myoglobin antibody [EP3081Y]
ab320144
Conjugated
Alexa Fluor® 488 Anti-Myoglobin antibody [EP3081Y]
ab320145
Conjugated
Alexa Fluor® 647 Anti-Myoglobin antibody [EP3081Y]
ab320146
Conjugated
Alexa Fluor® 555 Anti-Myoglobin antibody [EP3081Y]
ab320147
Conjugated
Alexa Fluor® 594 Anti-Myoglobin antibody [EP3081Y]
ab320148
Conjugated
PE Anti-Myoglobin antibody [EP3081Y]
ab320149
Conjugated
APC Anti-Myoglobin antibody [EP3081Y]
ab321732
Conjugated
Alexa Fluor® 750 Anti-Myoglobin antibody [EP3081Y]

Images

Western blot - Anti-Myoglobin antibody [EP3081Y] (AB77232), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Tested	Tested	Expected
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes Use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250 - 1/500	Notes Use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Species Human	Dilution info 1/250 - 1/500	Notes Use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

See full target information MB

Function

Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed:30918256, PubMed:34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed:32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed:34679218).

Alternative names

Myoglobin, Nitrite reductase MB, Pseudoperoxidase MB, MB

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP3081Y
Purification technique: Affinity purification Protein A
Dissociation constant: 1.78 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Myoglobin antibody [EP3081Y] (ab77232) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Myoglobin?

Anti-Myoglobin [EP3081Y] (ab77232) specifically detects a band for Myoglobin (UniProt: P02144) at a molecular weight of 17kDa.

Recommended positive controls

Human, rat and mouse muscle tissue lysates; human heart muscle tissue. C2C12 cell line.

Trusted by the scientific community

Anti-Myoglobin [EP3081Y] (ab77232) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EP3081Y] also available for your convenience:
ab77232, HRP - ab199243, Carrier free - ab232388, Alexa Fluor® 488 - ab320144, Alexa Fluor® 647 - ab320145, Alexa Fluor® 555 - ab320146, Alexa Fluor® 594 - ab320147, PE - ab320148, APC - ab320149, Alexa Fluor® 750 - ab321732

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Myoglobin also known as MB is a small globular protein with a molecular weight of approximately 17 kDa. It functions as an oxygen-binding protein and is expressed mainly in cardiac and skeletal muscle tissue where it facilitates oxygen storage and transport. The myoglobin protein plays an important role in maintaining the oxygen supply needed during muscular contraction and intense physical activity.

Biological function summary

Myoglobin in muscle cells acts to store oxygen which provides a rapid release when required during muscle contraction. Myoglobin serves as a monomer and does not form part of a complex. Its structure allows it to temporarily store and relay oxygen where it is most required enhancing the often abrupt demands of muscles for oxygen. The ability of myoglobin to bind oxygen and release it under hypoxic conditions is central to its biological role in vertebrates.

Pathways

The function of myoglobin in aerobic respiration in muscles involves its participation in the oxygen transport pathway. This protein closely interacts with hemoglobin to mobilize oxygen effectively to mitochondria during muscle contraction. Unlike hemoglobin myoglobin has a hyperbolic oxygen dissociation curve which allows it to provide oxygen at lower partial pressures contributing significantly to the efficient metabolism during hypoxia or intense muscular exertion.

Associated diseases and disorders

Myoglobin plays a significant role in conditions such as rhabdomyolysis and myocardial infarction. Rhabdomyolysis a syndrome caused by muscle injury results in the release of myoglobin into the bloodstream. Myoglobin detection kits including myoglobin ELISA are essential tools for the diagnosis of these conditions. Moreover its rapid increase in plasma levels after heart muscle damage enables its use as an early marker for myocardial infarction. Myoglobin's interaction with proteins like creatine kinase provides valuable information on muscle damage and cardiac events.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Myoglobin Western blot staining using rabbit Anti-Myoglobin antibody
All lanes: Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution
Lane 1: Human muscle lysate at 20 µg
Lane 2: Mouse muscle lysate at 20 µg
Lane 3: Rat muscle lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 1s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)and DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Unpurified ab77232 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This image is courtesy of an anonymous customer review
Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with unpurified ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4°C. A IRDye^® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.
All lanes: Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDa
Exposure time: 5min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Unpurified ab77232 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)
All lanes: Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution
Lane 1: Human heart muscle lysate at 10 µg
Lane 2: Mouse heart muscle lysate at 10 µg
Lane 3: Rat heart muscle lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Unpurified ab77232 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Unpurified ab77232 showing negative staining in Normal human liver tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)
Purified ab77232 staining Myoglobin in Mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
OI-RD Scanning - Anti-Myoglobin antibody [EP3081Y] (ab77232)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.