Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Unpurified ab77232 showing negative staining in Normal kidney tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Unpurified ab77232 showing positive staining in Skeletal muscle tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Unpurified ab77232 showing negative staining in Normal liver tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Unpurified ab77232 showing negative staining in Normal brain tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Purified ab77232 staining Myoglobin in mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A ready to use HRP-conjugated Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) and DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (AB232388)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.