Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on human tonsil tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322-9] (<a href='/en-us/products/primary-antibodies/myom1-antibody-epr17322-9-ab205618'>ab205618</a>) at 1/5000 dilution

Lane 1:

Rat heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322-9] (<a href='/en-us/products/primary-antibodies/myom1-antibody-epr17322-9-ab205618'>ab205618</a>) at 1/5000 dilution

All lanes:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 2min

• WB

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322-9] (<a href='/en-us/products/primary-antibodies/myom1-antibody-epr17322-9-ab205618'>ab205618</a>) at 1/5000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse muscle lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 5s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322-9] - BSA and Azide free (AB251439)

This data was developed using ab205618, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MYOM1 with ab205618 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human cardiac muscle tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.