Rabbit Recombinant Monoclonal MYOM1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img25582.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)")
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img23464.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)")
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img148269.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78163.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (AB251338)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
Alternative names
Myomesin-1, 190 kDa connectin-associated protein, 190 kDa titin-associated protein, Myomesin family member 1, MYOM1
Recommended products
Rabbit Recombinant Monoclonal MYOM1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17322
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251338 is the carrier-free version of Anti-MYOM1 antibody [EPR17322] ab201228.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MYOM1 also known as myomesin 1 is a protein with a molecular mass of approximately 185 kDa. It plays a mechanical role in muscle cells where it contributes to the M-band region of the sarcomere. MYOM1 is important in the structural integrity and elasticity of the myofibrils. Expression of this protein is specifically seen in cardiac and skeletal muscle tissues indicating its significance in muscle function.
- Biological function summary
The function of MYOM1 involves its contribution to sarcomere organization and stability. It acts as a scaffold in the M-band where it forms complexes with other sarcomeric proteins. These complexes include titin and muscle-specific kinase 1 which help maintain sarcomere structure during muscle contraction and stretch. MYOM1's interaction with these proteins is essential for muscle elasticity and proper muscle function.
- Pathways
MYOM1 operates within the muscle contraction pathway and the sarcomere organization pathway. It interacts closely with titin which is a giant protein essential for muscle elasticity and with beta-catenin a protein involved in cell signaling and cytoskeleton organization. The interaction and the pathways help maintain muscle function and structural integrity ensuring proper muscle responses to physiological demands.
- Associated diseases and disorders
MYOM1 is linked to the development of myopathies particularly myosin storage myopathy and hypertrophic cardiomyopathy. These conditions are associated with mutations or malfunctions in proteins such as titin and myosin which are part of MYOM1's network. Understanding the connection between MYOM1 and these proteins helps in unraveling the pathology of these muscle disorders presenting potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img25582.jpg "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MYOM1 antibody [EPR17322] (Anti-MYOM1 antibody [EPR17322] ab201228) at 1/20000 dilution
Lane 1: Mouse heart lysate at 20 µg
Lane 2: Mouse muscle lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 1min
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img23464.jpg "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MYOM1 antibody [EPR17322] (Anti-MYOM1 antibody [EPR17322] ab201228) at 1/20000 dilution
Lane 1: Rat heart lysate at 20 µg
Lane 2: Rat brain lysate at 20 µg
Lane 3: Rat kidney lysate at 20 µg
Lane 4: C6 (Rat glial tumor cells) whole cell lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 15s
![Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--western-blot-img148269.jpg "Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Western blot - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MYOM1 antibody [EPR17322] (Anti-MYOM1 antibody [EPR17322] ab201228) at 1/20000 dilution
All lanes: Human fetal heart tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 188 kDa
Observed band size: 188 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with Anti-MYOM1 antibody [EPR17322] ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78163.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with Anti-MYOM1 antibody [EPR17322] ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10476.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with Anti-MYOM1 antibody [EPR17322] ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104677.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)
This data was developed using Anti-MYOM1 antibody [EPR17322] ab201228, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with Anti-MYOM1 antibody [EPR17322] ab201228 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10476.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338), expandable thumbnail](https://content.abcam.com/products/images/myom1-antibody-epr17322-bsa-and-azide-free-ab251338--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104677.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] - BSA and Azide free (ab251338)")
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