Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Myosin (MYH1/2) with ab37484 at 3.228 μg/ml, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2μg/ml) (Green). Confocal image showing cytoplasmic staining in differentiated (treated) C2C12 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5μg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (4μg/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2μg/ml).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 μg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 μg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

• WB

Lab

Western blot - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

The expression profile observed is consistent with what has been described in the literature (PMID : 26010876).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myosin heavy chain antibody [A4.1025] (<a href='/en-us/products/primary-antibodies/myosin-heavy-chain-antibody-a41025-ab37484'>ab37484</a>) at 0.807 µg/mL

Lane 1:

C2C12 (mouse myoblasts) whole cell lysate at 20 µg

Lane 2:

Differentiated C2C12 whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa

Observed band size: 223 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

Exposure times : Lane 1 : 3 secs; Lane 2 : 1 sec.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myosin heavy chain antibody [A4.1025] (<a href='/en-us/products/primary-antibodies/myosin-heavy-chain-antibody-a41025-ab37484'>ab37484</a>) at 0.807 µg/mL

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa

Observed band size: 223 kDa

false

• WB

Lab

Western blot - Anti-Myosin heavy chain antibody [A4.1025] - BSA and Azide free (AB264490)

The extra bands under 223KDa were mainly caused by degradation (PMID : 3295257).

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab37484).

All lanes:

Western blot - Anti-Myosin heavy chain antibody [A4.1025] (<a href='/en-us/products/primary-antibodies/myosin-heavy-chain-antibody-a41025-ab37484'>ab37484</a>) at 0.1614 µg/mL

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa

Observed band size: 223 kDa

false

Exposure time: 3s