AB48003

Anti-Myosin Light Chain 2 antibody

Name: Anti-Myosin Light Chain 2 antibody. Rabbit polyclonal (ab48003) | Abcam
Brand: Abcam Limited
SKU: AB48003
Price: 565 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(19 Publications)

Rabbit Polyclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human MYL2 aa 1-50.

View Alternative Names

MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB48003)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB48003)

ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Unknown

Western blot - Anti-Myosin Light Chain 2 antibody (AB48003)

Arrowheads indicate the detection of both monophosphorylated (upper) and unphosphorylated (lower) forms of the protein.

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution

Lane 1:

Vascular myosin (rat aorta), loaded with 35 µg of lysate

Lane 2:

Cardiac myosin (mouse heart, loaded with 35 µg of lysate

Secondary

All lanes:

1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]

Predicted band size: 19 kDa

Observed band size: 19 kDa,24 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, ELISA, WB

applications

Immunogen

Synthetic Peptide within Human MYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P10916

Specificity

ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.

Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.

Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contractile protein that plays a role in heart development and function (PubMed : 23365102, PubMed : 32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

See full target information MYL2

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:1654087 PubMed40838175

2025

Maxim. Alleviates Primary Dysmenorrhea in a Mouse Model by Modulating Myometrial Contractions via NF-κB/COX-2 Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Soo Kim,Kang-In Lee,Heung Joo Yuk,Yousang Jo,Hyungjun Kim,Ki-Sun Park

Journal of applied physiology (Bethesda, Md. : 1985) 136:764-773 PubMed38328824

2024

Ablation of skeletal muscle estrogen receptor alpha impairs contractility in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Brian P Sullivan,Brittany C Collins,Shawna L McMillin,Elise Toussaint,Clara Z Stein,Espen E Spangenburg,Dawn A Lowe

iScience 26:108466 PubMed38077137

2023

Robust small molecule-aided cardiac reprogramming systems selective to cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmeng Tao,Yang Yang,Zhenghao Yang,Lipeng Wang,Shi-Qiang Wang,Yang Zhao

Cell stem cell 27:50-63.e5 PubMed32619518

2020

Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jan W Buikema,Soah Lee,William R Goodyer,Renee G Maas,Orlando Chirikian,Guang Li,Yi Miao,Sharon L Paige,Daniel Lee,Haodi Wu,David T Paik,Siyeon Rhee,Lei Tian,Francisco X Galdos,Nazan Puluca,Benjamin Beyersdorf,James Hu,Aimee Beck,Sneha Venkamatran,Srilatha Swami,Paul Wijnker,Maike Schuldt,Larissa M Dorsch,Alain van Mil,Kristy Red-Horse,Joy Y Wu,Caroline Geisen,Michael Hesse,Vahid Serpooshan,Stefan Jovinge,Bernd K Fleischmann,Pieter A Doevendans,Jolanda van der Velden,K Christopher Garcia,Joseph C Wu,Joost P G Sluijter,Sean M Wu

PLoS genetics 16:e1008639 PubMed32453731

2020

Novel frameshift variant in MYL2 reveals molecular differences between dominant and recessive forms of hypertrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sathiya N Manivannan,Sihem Darouich,Aida Masmoudi,David Gordon,Gloria Zender,Zhe Han,Sara Fitzgerald-Butt,Peter White,Kim L McBride,Maher Kharrat,Vidu Garg

Nature communications 10:2797 PubMed31243273

2019

Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

JinSeok Park,Deok-Ho Kim,Sagar R Shah,Hong-Nam Kim, Kshitiz,Peter Kim,Alfredo Quiñones-Hinojosa,Andre Levchenko

Journal of cell science 131: PubMed29848657

2018

A two-phase response of endothelial cells to hydrostatic pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Prystopiuk,Benedikt Fels,Caroline Sophie Simon,Ivan Liashkovich,Dzmitry Pasrednik,Cornelius Kronlage,Roland Wedlich-Söldner,Hans Oberleithner,Johannes Fels

Journal of applied physiology (Bethesda, Md. : 198 124:980-992 PubMed29345963

2018

Deletion of estrogen receptor α in skeletal muscle results in impaired contractility in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany C Collins,Tara L Mader,Christine A Cabelka,Melissa R Iñigo,Espen E Spangenburg,Dawn A Lowe

Molecular biology of the cell 28:771-782 PubMed28077619

2017

Relative importance of β- and γ-actin in primary mouse embryonic fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobai Patrinostro,Allison R O'Rourke,Christopher M Chamberlain,Branden S Moriarity,Benjamin J Perrin,James M Ervasti

Journal of neurochemistry 138:60-73 PubMed27062289

2016

Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Xiaomei Wu,Hai-Yuan Yue,Yong-Chuan Zhu,Jianhua Xu

View all publications

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Product promise

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Anti-Myosin Light Chain 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB48003)

Western blot - Anti-Myosin Light Chain 2 antibody (AB48003)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise