Rabbit Polyclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human MYL2 aa 1-50.
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Publications
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- Cell stem cell 27:50-63.e52020Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJan W Buikema,Soah Lee,William R Goodyer,Renee G Maas,Orlando Chirikian,Guang Li,Yi Miao,Sharon L Paige,Daniel Lee,Haodi Wu,David T Paik,Siyeon Rhee,Lei Tian,Francisco X Galdos,Nazan Puluca,Benjamin Beyersdorf,James Hu,Aimee Beck,Sneha Venkamatran,Srilatha Swami,Paul Wijnker,Maike Schuldt,Larissa M Dorsch,Alain van Mil,Kristy Red-Horse,Joy Y Wu,Caroline Geisen,Michael Hesse,Vahid Serpooshan,Stefan Jovinge,Bernd K Fleischmann,Pieter A Doevendans,Jolanda van der Velden,K Christopher Garcia,Joseph C Wu,Joost P G Sluijter,Sean M WuPubMed 32619518
- PLoS genetics 16:e10086392020Novel frameshift variant in MYL2 reveals molecular differences between dominant and recessive forms of hypertrophic cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSathiya N Manivannan et. al.PubMed 32453731
- Nature communications 10:27972019Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinSeok Park et. al.PubMed 31243273
- Journal of cell science 131:2018A two-phase response of endothelial cells to hydrostatic pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria Prystopiuk et. al.PubMed 29848657
- Journal of applied physiology (Bethesda, Md. : 198 124:980-9922018Deletion of estrogen receptor α in skeletal muscle results in impaired contractility in female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany C Collins et. al.PubMed 29345963
- Molecular biology of the cell 28:771-7822017Relative importance of β- and γ-actin in primary mouse embryonic fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobai Patrinostro et. al.PubMed 28077619
- Journal of neurochemistry 138:60-732016Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Li et. al.PubMed 27062289
- American journal of physiology. Endocrinology and 310:E724-332016Estradiol modulates myosin regulatory light chain phosphorylation and contractility in skeletal muscle of female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaojuan Lai et. al.PubMed 26956186
- The Journal of biological chemistry 290:4663-46762014Regulation of gene transcription by voltage-gated L-type calcium channel, Cav1.3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Lu,Padmini Sirish,Zheng Zhang,Ryan L Woltz,Ning Li,Valeriy Timofeyev,Anne A Knowlton,Xiao-Dong Zhang,Ebenezer N Yamoah,Nipavan ChiamvimonvatPubMed 25538241
- Bioscience reports 34:2014Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshling Holland et. al.PubMed 24895011
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P10916
Reactivity data
Select an application| IHC-P | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Zebrafish, Pig | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Zebrafish, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Zebrafish, Pig | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Zebrafish, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
Alternative names
MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v
Recommended products
Rabbit Polyclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human MYL2 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P10916
- Purification technique
- Affinity purification Immunogen
- Specificity
ab48003 is directed against the regulatory light chain of smooth and non muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.
Cross reactivity is expected with myosin light chain from human, mouse and rat sources. Reactivity with the protein from other species has not been determined; however, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
- Biological function summary
Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.
- Pathways
Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.
- Associated diseases and disorders
Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Myosin Light Chain 2 antibody (ab48003)
Arrowheads indicate the detection of both monophosphorylated (upper) and unphosphorylated (lower) forms of the protein.
All lanes: Western blot - Anti-Myosin Light Chain 2 antibody (ab48003) at 1/600 dilution
Lane 1: Vascular myosin (rat aorta), loaded with 35 µg of lysate
Lane 2: Cardiac myosin (mouse heart, loaded with 35 µg of lysate
SecondaryAll lanes: 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L]
Predicted band size: 19 kDa
Observed band size: 19 kDa, 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab48003)
ab48003 (2µg/ml) staining Myosin Light Chain 2 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cell cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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