Anti-Myosin Light Chain 2 antibody (ab79935) is a rabbit polyclonal antibody detecting Myosin Light Chain 2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.
- Over 30 publications
- Trusted since 2009
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- Scientific reports 13:206852023Biophysical properties of Na1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles-Albert Chapotte-Baldacci,Marion Pierre,Mohammed Djemai,Valérie Pouliot,Mohamed ChahinePubMed 38001331
- NPJ Regenerative medicine 8:262023Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynn Yap,Li Yen Chong,Clarissa Tan,Swarnaseetha Adusumalli,Millie Seow,Jing Guo,Zuhua Cai,Sze Jie Loo,Eric Lim,Ru San Tan,Elina Grishina,Poh Loong Soong,Narayan Lath,Lei Ye,Enrico Petretto,Karl TryggvasonPubMed 37236990
- eLife 12:2023Molecular characterization of the intact mouse muscle spindle using a multi-omics approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBavat Bornstein,Lia Heinemann-Yerushalmi,Sharon Krief,Ruth Adler,Bareket Dassa,Dena Leshkowitz,Minchul Kim,Guy Bewick,Robert W Banks,Elazar ZelzerPubMed 36744866
- Environmental health perspectives 130:1170022022Cardiac Development in the Presence of Cadmium: An Study Using Human Embryonic Stem Cells and Cardiac Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian Wu,Yichang Chen,Anthony Luz,Guang Hu,Erik J TokarPubMed 36321828
- Journal of tissue engineering 13:204173142211279082022Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiuru Li,Alexandra Wiesinger,Lianne Fokkert,Bastiaan J Boukens,Arie O Verkerk,Vincent M Christoffels,Gerard J J Boink,Harsha D DevallaPubMed 36277058
- iScience 25:1049712022A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHunter J Johnson,Saheli Chakraborty,Riya J Muckom,Nitash P Balsara,David V SchafferPubMed 36147944
- Cell reports 40:1111462022Cardiac ultrastructure inspired matrix induces advanced metabolic and functional maturation of differentiated human cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunaid Afzal,Yamin Liu,Wenqiang Du,Yasir Suhail,Pengyu Zong,Jianlin Feng,Visar Ajeti,Wasim A Sayyad,Joerg Nikolaus,Maya Yankova,Alix C Deymier,Lixia Yue, KshitizPubMed 35905711
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e22022822022A Uterus-Inspired Niche Drives Blastocyst Development to the Early Organogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Gu,Jia Guo,Jinglei Zhai,Guihai Feng,Xianning Wang,Zili Gao,Kai Li,Shen Ji,Leyun Wang,Yanhong Xu,Xi Chen,Yiming Wang,Shanshan Guo,Man Yang,Linlin Li,Hua Han,Liyuan Jiang,Yongqiang Wen,Liu Wang,Jie Hao,Wei Li,Shutao Wang,Hongmei Wang,Qi GuPubMed 35843885
- International journal of molecular sciences 23:2022Dysregulation of the Enteric Nervous System in the Mid Colon of Complement Component 3 Knockout Mice with Constipation Phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Ju Choi,Hee Jin Song,Ji Eun Kim,Su Jin Lee,You Jeong Jin,Yu Jeong Roh,Ayun Seol,Hye Sung Kim,Dae Youn HwangPubMed 35743302
- Science advances 8:eabj66212022Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyosuk Kim,Byeong-Wook Song,Soon-Jung Park,Seong Woo Choi,Hanbyeol Moon,Ki-Chul Hwang,Sun-Woong Kang,Sung-Hwan Moon,Yoosoo Yang,Ick Chan Kwon,Sun Hwa KimPubMed 35213232
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Human | Dilution info 5 µg/mL | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
Alternative names
MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v
Recommended products
Anti-Myosin Light Chain 2 antibody (ab79935) is a rabbit polyclonal antibody detecting Myosin Light Chain 2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.
- Over 30 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Myosin Light Chain 2 antibody (ab79935) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of Myosin Light Chain 2?
Anti-Myosin Light Chain 2 (ab79935) specifically detects a band for Myosin Light Chain 2 (UniProt: P10916) at a molecular weight of 19kDa.
Trusted by the scientific community
Anti-Myosin Light Chain 2 (ab79935) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
- Biological function summary
Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.
- Pathways
Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.
- Associated diseases and disorders
Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)
All lanes: Western blot - Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/mL
Lane 1: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 2: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Lane 3: Heart (Mouse) Tissue Lysate at 10 µg
Lane 4: Heart (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 17 kDa, 19 kDa, 8 kDa
Exposure time: 15min
Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5μg of Rabbit polyclonal to Myosin Light Chain 2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 19kDa; Myosin Light Chain 2
All lanes: Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 20min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)
IHC image of Myosin light chain 2 antibody staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab79935, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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