AB79935

Anti-Myosin Light Chain 2 antibody

Name: Anti-Myosin Light Chain 2 antibody (ab79935) | Abcam
Brand: Abcam Limited
SKU: AB79935
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

BOND RX™ Validated
What is this?

(2 Reviews)

(58 Publications)

Anti-Myosin Light Chain 2 antibody (ab79935) is a rabbit polyclonal antibody detecting Myosin Light Chain 2 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Over 30 publications
- Trusted since 2009

View Alternative Names

MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

3 Images

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Western blot - Anti-Myosin Light Chain 2 antibody (AB79935)

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 3:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 4:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 19 kDa

Observed band size: 17 kDa,19 kDa,8 kDa

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Exposure time: 15min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB79935)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB79935)

IHC image of Myosin light chain 2 antibody staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BOND^TM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab79935, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (AB79935)

Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5μg of Rabbit polyclonal to Myosin Light Chain 2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.

Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

Band : 19kDa; Myosin Light Chain 2

All lanes:

Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)

Predicted band size: 19 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Myosin Light Chain 2 antibody (ab79935) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Myosin Light Chain 2?
Anti-Myosin Light Chain 2 (ab79935) specifically detects a band for Myosin Light Chain 2 (UniProt: P10916) at a molecular weight of 19kDa.

Trusted by the scientific community
Anti-Myosin Light Chain 2 (ab79935) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.

Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contractile protein that plays a role in heart development and function (PubMed : 23365102, PubMed : 32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

See full target information MYL2

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Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30648 PubMed40835711

2025

Effect of mechanical stretching stimulation on maturation of human iPS cell-derived cardiomyocytes co-cultured with human gingival fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxue Wang,Harumi Idei,Chen Wang,Yin Liang,Yun Liu,Yusuke Matsuda,Ken Takahashi,Hiroshi Kamioka,Keiji Naruse

iScience 28:112843 PubMed40662194

2025

Serine synthesis pathway regulates cardiac differentiation from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohiko C Umei,Shugo Tohyama,Yuika Morita-Umei,Manami Katoh,Seitaro Nomura,Kotaro Haga,Takako Hishiki,Tomomi Matsuura,Hidenori Tani,Yusuke Soma,Otoya Sekine,Masatoshi Ohno,Masashi Nakamura,Taijun Moriwaki,Yoshikazu Kishino,Keiichi Fukuda,Masaki Ieda

PloS one 20:e0326312 PubMed40549735

2025

Prostaglandin E2 dependent migration of human brain endothelial cells is mediated through Rho-Kinase-II.

Applications

Unspecified application

Species

Unspecified reactive species

Gausal Azam Khan,Arjun Ghosh

The EMBO journal 44:767-780 PubMed39707024

2024

Mechanical signaling through membrane tension induces somal translocation during neuronal migration.

Applications

Unspecified application

Species

Unspecified reactive species

Takunori Minegishi,Honami Hasebe,Tomoya Aoyama,Keiji Naruse,Yasufumi Takahashi,Naoyuki Inagaki

Nature communications 15:10206 PubMed39604360

2024

Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amos A Lim,Delaram Pouyabahar,Mishal Ashraf,Kate Huang,Michelle Lohbihler,Brandon M Murareanu,Matthew L Chang,Maggie Kwan,Faisal J Alibhai,Thinh Tran,Amine Mazine,Michael A Laflamme,Gary D Bader,Zachary Laksman,Stephanie Protze

iScience 27:111234 PubMed39569381

2024

Metabolic changes of human induced pluripotent stem cell-derived cardiomyocytes and teratomas after transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Soma,Shugo Tohyama,Akiko Kubo,Tomoteru Yamasaki,Noriko Kabasawa,Kotaro Haga,Hidenori Tani,Yuika Morita-Umei,Tomohiko C Umei,Otoya Sekine,Masashi Nakamura,Taijun Moriwaki,Sho Tanosaki,Shota Someya,Yujiro Kawai,Masatoshi Ohno,Yoshikazu Kishino,Hideaki Kanazawa,Jun Fujita,Ming-Rong Zhang,Makoto Suematsu,Keiichi Fukuda,Masaki Ieda

STAR protocols 5:103452 PubMed39520685

2024

Protocol for quantifying murine cardiomyocyte cell division by single-cell suspension.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Swift,Alexandra L Purdy,Michaela Patterson

Cell reports methods 4:100860 PubMed39255794

2024

A broadly applicable method for quantifying cardiomyocyte cell division identifies proliferative events following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Swift,Alexandra L Purdy,Tyler Buddell,Jerrell J Lovett,Smrithi V Chanjeevaram,Anooj Arkatkar,Caitlin C O'Meara,Michaela Patterson

Cell stem cell 31:1222-1238.e10 PubMed38908380

2024

Primitive macrophages enable long-term vascularization of human heart-on-a-chip platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Shira Landau,Yimu Zhao,Homaira Hamidzada,Gregory M Kent,Sargol Okhovatian,Rick Xing Ze Lu,Chuan Liu,Karl T Wagner,Krisco Cheung,Sarah A Shawky,Daniel Vosoughi,Erika Leigh Beroncal,Ian Fernandes,Carolyn L Cummins,Ana C Andreazza,Gordon M Keller,Slava Epelman,Milica Radisic

American journal of physiology. Heart and circulatory physiology 327:H377-H389 PubMed38847758

2024

is sufficient but not required for cardiomyocyte cell-cycle activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaelin A Akins,Michael A Flinn,Samantha K Swift,Smrithi V Chanjeevaram,Alexandra L Purdy,Tyler Buddell,Mary E Kolell,Kaitlyn G Andresen,Samantha Paddock,Sydney L Buday,Matthew B Veldman,Caitlin C O'Meara,Michaela Patterson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin Light Chain 2 antibody

Western blot - Anti-Myosin Light Chain 2 antibody (AB79935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB79935)

Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (AB79935)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (58)

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