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AB89594

Anti-Myosin Light Chain 2 antibody [AT3B2]

5

(3 Reviews)

|

(8 Publications)

Mouse Monoclonal Myosin Light Chain 2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYL2.

View Alternative Names

MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

3 Images
Flow Cytometry (Intracellular) - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)

Overlay histogram showing A431 cells stained with ab89594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89594, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This image was generated using the ascites version of the product.

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)
  • WB

AbReview34713****

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/2000 dilution

All lanes:

Rabbit heart tissue lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-mouse at 1/5000 dilution

Predicted band size: 19 kDa

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Exposure time: 1min

This image is courtesy of an anonymous Abreview

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)
  • WB

Unknown

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/1000 dilution

All lanes:

rat heart extracts at 60 µg

Secondary

All lanes:

goat anti-mouse HRP

Predicted band size: 19 kDa

Observed band size: 19 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AT3B2

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MYL2.

P10916

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Anti-Cardiac Troponin T antibody [EPR20266]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (AB209813)

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  • 2
Primary Antibodies

AB2480

Anti-MYL12A (phospho S19) antibody

Western blot - Anti-MYL12A (phospho S19) antibody (AB2480)

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Primary Antibodies

AB308066

Anti-RNF5/NG2 antibody [EPR27133-30]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] (AB308066)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.2% Sodium azide Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contractile protein that plays a role in heart development and function (PubMed : 23365102, PubMed : 32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
See full target information MYL2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 12:e027769 PubMed36802924

2023

Role of PARP and TRPM2 in VEGF Inhibitor-Induced Vascular Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Karla B Neves,Rheure Alves-Lopes,Augusto C Montezano,Rhian M Touyz

Scientific reports 11:17168 PubMed34433864

2021

Na1.5 knockout in iPSCs: a novel approach to study Na1.5 variants in a human cardiomyocyte environment.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Pierre,Mohammed Djemai,Hugo Poulin,Mohamed Chahine

Scientific reports 11:13866 PubMed34230586

2021

Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Sapp,Aitor Aguirre,Gayatri Mainkar,Jeffrey Ding,Eric Adler,Ronglih Liao,Sonia Sharma,Mohit Jain

Biology open 7: PubMed30545950

2018

Dynamic microtubules drive fibroblast spreading.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tvorogova,Aleena Saidova,Tatiana Smirnova,Ivan Vorobjev

Developmental cell 47:439-452.e6 PubMed30318244

2018

A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bipul R Acharya,Alexander Nestor-Bergmann,Xuan Liang,Shafali Gupta,Kinga Duszyc,Estelle Gauquelin,Guillermo A Gomez,Srikanth Budnar,Philippe Marcq,Oliver E Jensen,Zev Bryant,Alpha S Yap

Disease models & mechanisms 11: PubMed29752279

2018

A comprehensive study of calcific aortic stenosis: from rabbit to human samples.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Mourino-Alvarez,Montserrat Baldan-Martin,Tamara Sastre-Oliva,Marta Martin-Lorenzo,Aroa Sanz Maroto,Nerea Corbacho-Alonso,Raul Rincon,Tatiana Martin-Rojas,Luis Fernando Lopez-Almodovar,Gloria Alvarez-Llamas,Fernando Vivanco,Luis Rodriguez Padial,Fernando de la Cuesta,Maria Gonzalez Barderas

BioResearch open access 4:374-88 PubMed26487982

2015

Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Valerio Russo,Ehsan Omidi,Abbas Samani,Andrew Hamilton,Lauren E Flynn

Cell cycle (Georgetown, Tex.) 13:3450-9 PubMed25485589

2014

The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling.

Applications

Unspecified application

Species

Human

Ryan D Beveridge,Christopher J Staples,Abhijit A Patil,Katie N Myers,Sarah Maslen,J Mark Skehel,Simon J Boulton,Spencer J Collis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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