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Mouse Monoclonal Myosin Light Chain 2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYL2.


Images

Flow Cytometry (Intracellular) - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594), expandable thumbnail
  • Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594), expandable thumbnail
  • Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (AB89594), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.2% Sodium azide
Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human MYL2. Database link P10916

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Expected
Tested
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

Alternative names

MLC2, MYL2, MLC2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

Recommended products

Mouse Monoclonal Myosin Light Chain 2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MYL2.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human MYL2. Database link P10916
Clone number

AT3B2

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.

Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Associated diseases and disorders

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.

Product promise

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3 product images

  • Flow Cytometry (Intracellular) - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)

    Overlay histogram showing A431 cells stained with ab89594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89594, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/2000 dilution

    All lanes: Rabbit heart tissue lysate at 10 µg

    Secondary

    All lanes: HRP conjugated goat anti-mouse at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Exposure time: 1min

  • Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594), expandable thumbnail

    Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [AT3B2] (ab89594) at 1/1000 dilution

    All lanes: rat heart extracts at 60 µg

    Secondary

    All lanes: goat anti-mouse HRP

    Developed using the ECL technique.

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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