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Rabbit Recombinant Monoclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 36 publications.


Images

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721), expandable thumbnail
  • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Rat
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/600
Notes

The use of an HRP/AP polymerized secondary antibody is recommended.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Rat
Dilution info
1/10000
Notes

-

Species
Human
Dilution info
1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

Alternative names

MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

Recommended products

Rabbit Recombinant Monoclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 36 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3741
Purification technique
Affinity purification Protein A
Dissociation constant
2.78 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.

Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Associated diseases and disorders

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

    Lane 1: Human heart lysate

    Lane 2: Human skeletal muscle lysate

    Lane 3: Mouse heart lysate

    Lane 4: Rat heart lysate

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Myosin light chain-2 (MYL2, also called MLC-2) is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle (PMID: 26074085). Although some cell lines are reported to be MYL2 positive, such as HeLa, 293T and MCF7 (PMID: 21487036, 21487036, 23093227), ab92721 only works in muscle and heart tissues according to our internal testing.

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 20 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

    Exposure time: 10s

  • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

    Lane 1: Western blot - Recombinant Human Myosin Light Chain 2 protein (Recombinant Human Myosin Light Chain 2 protein ab79185) at 0.1 µg

    Lane 2: Western blot - Recombinant Human Myosin Light Chain 2 protein (Recombinant Human Myosin Light Chain 2 protein ab79185) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Exposure time: 4min

  • Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721), expandable thumbnail

    Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

    All lanes: Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

    Lane 1: Western blot - Recombinant Human Myosin Light Chain 2 protein (Recombinant Human Myosin Light Chain 2 protein ab79185) at 0.1 µg

    Lane 2: Western blot - Recombinant Human Myosin Light Chain 2 protein (Recombinant Human Myosin Light Chain 2 protein ab79185) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Exposure time: 8min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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