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AB92721

Anti-Myosin Light Chain 2 antibody [EPR3741]

3

(5 Reviews)

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(42 Publications)

Rabbit Recombinant Monoclonal Myosin Light Chain 2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 42 publications.

View Alternative Names

MLC2, MYL2, MLC-2, MLC-2v, Cardiac myosin light chain 2, Ventricular myosin light chain 2, MLC-2s/v

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. M3

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. M3

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Myosin Light Chain 2 with Purified ab92721 at 1/600 dilution (0.16 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. M3

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • WB

Unknown

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

Lane 1:

Human heart lysate at 15 µg

Lane 2:

Human skeletal muscle lysate at 15 µg

Lane 3:

Mouse heart lysate at 20 µg

Lane 4:

Rat heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • WB

Lab

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

Myosin light chain-2 (MYL2, also called MLC-2) is an ~19kDa sarcomeric protein that belongs to the EF-hand calcium binding protein superfamily and exists as three major isoforms encoded by three distinct genes in mammalian striated muscle (PMID : 26074085). Although some cell lines are reported to be MYL2 positive, such as HeLa, 293T and MCF7 (PMID : 21487036, 21487036, 23093227), ab92721 only works in muscle and heart tissues according to our internal testing.

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Exposure time: 10s

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • WB

Unknown

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

Lane 1:

Western blot - Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-myosin-light-chain-2-protein-ab79185'>ab79185</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-myosin-light-chain-2-protein-ab79185'>ab79185</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 4min

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)
  • WB

Unknown

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (AB92721)

All lanes:

Western blot - Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

Lane 1:

Western blot - Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-myosin-light-chain-2-protein-ab79185'>ab79185</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant Human Myosin Light Chain 2 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-myosin-light-chain-2-protein-ab79185'>ab79185</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 8min

  • Carrier free

    Anti-Myosin Light Chain 2 antibody [EPR3741] - BSA and Azide free

  • 660 APC

    APC Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 578 PE

    PE Anti-Myosin Light Chain 2 antibody [EPR3741]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Myosin Light Chain 2 antibody [EPR3741]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3741

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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AB79935

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BOND RX™ Validated

Western blot - Anti-Myosin Light Chain 2 antibody (AB79935)

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Primary Antibodies

AB48003

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (AB48003)

  • 4
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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "<p>The use of an HRP/AP polymerized secondary antibody is recommended.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin Light Chain 2 (MLC2) also known as ventricular myosin regulatory light chain (MLC2v) or smooth muscle myosin light chain is a critical component of the myosin protein complex. It is a low-molecular-weight protein with a mass typically around 20 kDa. Myosin Light Chain 2 plays a mechanical role in muscle contraction where it interacts with myosin heavy chain and actin filaments. This interaction modulates muscle contraction in both cardiac and smooth muscle tissues. MLC2 is ubiquitously expressed in muscles but exhibits specific expression patterns in cardiac and smooth muscles.
Biological function summary

Myosin Light Chain 2 plays a fundamental role in muscle function by regulating myofilament sensitivity to calcium ions. It functions as part of the myosin complex which is essential for actin-myosin interactions required for muscle contraction. Phosphorylation of MLC2 alters the actomyosin activity affecting muscle contraction strength and speed. This regulation contributes significantly to muscle tone contractility and overall heart function.

Pathways

Myosin Light Chain 2 is a pivotal player in the calcium signaling and smooth muscle contraction pathways. Within these pathways phosphorylation leads to myosin cross-bridge cycling with actin facilitated by proteins like calmodulin and myosin light chain kinase (MLCK). This biochemical interaction not only underpins smooth muscle function but also plays roles in other processes like cell motility and division emphasizing MLC2's versatility in cellular mechanics.

Aberrations in Myosin Light Chain 2 function or expression implicate it in cardiac hypertrophy and certain smooth muscle dysfunctions such as hypertension. Changes in MLC2 phosphorylation state can directly affect cardiac contractility worsening conditions like hypertrophic cardiomyopathy. It also relates closely with other proteins such as MLCK and calmodulin which are involved in these disease pathways highlighting a complex network of regulatory mechanisms that when disrupted can lead to severe health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contractile protein that plays a role in heart development and function (PubMed : 23365102, PubMed : 32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).
See full target information MYL2
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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:519 PubMed41013775

2025

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yukihiro Saito,Kazufumi Nakamura,Yuki Katanosaka,Toshihiro Iida,Dai Kusumoto,Ryushi Sato,Riki Adachi,Satoshi Shimizu,Junko Kurokawa,Satoshi Akagi,Masashi Yoshida,Toru Miyoshi,Hiroshi Morita,Keiji Naruse,Mikako Nishida,Heiichiro Udono,Jianhua Zhang,Shinsuke Yuasa,Timothy J Kamp,Hiroshi Ito

Frontiers in pharmacology 16:1573483 PubMed40520190

2025

Trifolin inhibits the calcium-driven contraction pathway in vascular smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Renfeng Li,Jinkong Wu,Meizhu Wu,Farman Ali,Yanyan Yang,Hong Chen,Zhi Guo,Dawei Lian,Aling Shen,Jun Peng

PloS one 20:e0316607 PubMed39804876

2025

Increased resting heart rate indicates high-workload hearts with augmented aortic hydraulic power in hypertensive pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Pao-Yen Lin,Bo-Wen Lin,Tong-Sian Lai,Yan-Hsiang Yang,Meei Jyh Jiang

Cardiovascular toxicology 25:34-47 PubMed39521734

2024

Isoprenaline Inhibits Histone Demethylase LSD1 to Induce Cardiac Hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Wu,Bo Yang,Yingze Sun,Guanwei Fan,Lina Ma,Ying Ma,Xianjia Xiong,Hui Zhou,Huiping Wang,Ling Zhang,Bing Yang

iScience 27:109156 PubMed38439960

2024

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Zhang,Sisi Chen,Bin Ma,Yingmei Ding,Xiaofen Liu,Caijun He,Biao Wang,Mei Yuan

Frontiers in cardiovascular medicine 10:1261330 PubMed37745108

2023

tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Yukihiro Saito,Naoko Nose,Toshihiro Iida,Kaoru Akazawa,Takayuki Kanno,Yuki Fujimoto,Takanori Sasaki,Masaru Akehi,Takahiro Higuchi,Satoshi Akagi,Masashi Yoshida,Toru Miyoshi,Hiroshi Ito,Kazufumi Nakamura

The Journal of clinical investigation 133: PubMed37200096

2023

1-Deoxynojirimycin promotes cardiac function and rescues mitochondrial cristae in mitochondrial hypertrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Zhuang,Fengfeng Guo,Lei Fu,Yufei Dong,Shaofang Xie,Xue Ding,Shuangyi Hu,Xuanhao D Zhou,Yangwei Jiang,Hui Zhou,Yue Qiu,Zhaoying Lei,Mengyao Li,Huajian Cai,Mingjie Fan,Lingjie Sang,Yong Fu,Dong Zhang,Aifu Lin,Xu Li,Tilo Kunath,Ruhong Zhou,Ping Liang,Zhong Liu,Qingfeng Yan

Journal of cancer research and clinical oncology 149:7837-7848 PubMed37036506

2023

The adverse effect of anticancer drug toremifene on vascular smooth muscle cells is an important aspect of its tumor growth inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyun Wang,Juan Liu,Mingxing Wang,Chun Yang,Guixue Wang,Tingzhang Hu

Proceedings of the National Academy of Sciences of the United States of America 120:e2222005120 PubMed36913580

2023

In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Chandler,Conor Treacy,Simon Ameer-Beg,Elisabeth Ehler,Malcolm Irving,Thomas Kampourakis

Molecules (Basel, Switzerland) 27: PubMed35164001

2022

Anti-Cancer Effects of α-Cubebenoate Derived from in CT26 Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Eun Gong,Ji Eun Kim,Su Jin Lee,Yun Ju Choi,You Jeong Jin,Young Whan Choi,Sun Il Choi,Dae Youn Hwang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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