AB233220

Anti-Myosin light chain 3 antibody

Name: Anti-Myosin light chain 3 antibody (ab233220) | Abcam
Brand: Abcam Limited
SKU: AB233220
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal MYL3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYL3 aa 1 to C-terminus.

View Alternative Names

Myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, CMLC1, MLC1SB, VLCl, MLC-lV/sb, MYL3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody (AB233220)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody (AB233220)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Slow skeletal Myosin using ab233220 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

All lanes:

Western blot - Anti-Myosin light chain 3 antibody (ab233220) at 3 µg/mL

All lanes:

Mouse heart lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

All lanes:

Western blot - Anti-Myosin light chain 3 antibody (ab233220) at 3 µg/mL

All lanes:

Pig heart lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

All lanes:

Western blot - Anti-Myosin light chain 3 antibody (ab233220) at 3 µg/mL

All lanes:

Recombinant human Slow skeletal Myosin protein

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

All lanes:

Western blot - Anti-Myosin light chain 3 antibody (ab233220) at 3 µg/mL

All lanes:

Pig skeletal muscle lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MYL3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08590

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin light chain 3 (MLC3) also known as myosin regulatory light chain 3 is a part of the myosin protein complex. It plays a major role in muscle contraction by regulating the ATPase activity of myosin heads. MLC3 is encoded by the MYL3 gene and has a molecular mass of approximately 18kDa. It is expressed mainly in cardiac and skeletal muscle tissues. By interacting with myosin heavy chains MLC3 contributes to the force-generating capacity of muscles.

Biological function summary

MLC3 functions as an essential component of the myosin light chain complex. This complex regulates contractile activity in muscle cells by modulating the interaction between actin and myosin chains. MLC3 activates the mechanical movement of myosin chains impacting cardiac and skeletal muscle contraction. When activated it affects the speed and strength of muscle contraction and it also has a role in muscle differentiation and development.

Pathways

MLC3 is an integral part of the muscle contraction pathway. It interacts with proteins such as tropomyosin and troponin which are important for the regulation of muscle contraction. Additionally MLC3 participates in the calcium signaling pathway which is pivotal for contraction processes in muscles. This interaction helps to control the cytosolic calcium concentration a critical factor in muscle contraction dynamics.

MLC3 has connections to cardiomyopathy and hypertrophic conditions. Mutations or dysregulation of MYL3 can lead to cardiac hypertrophy a disease where the heart muscle thickens abnormally. MLC3's interaction with troponin and other myosin chains can exacerbate cardiac conditions when these pathways are disrupted. Studies have linked deficiencies in MLC3 to improper muscle development which may underline some muscle myopathies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Regulatory light chain of myosin. Does not bind calcium.

See full target information MYL3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced materials (Deerfield Beach, Fla.) 35:e2302801 PubMed37589156

2023

Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyi Huang,Yawei Du,Yanjia Chen,Zeping Qiu,Zhiyan Wang,Yingze Fan,Yunjing Shi,Zhuojin Li,Ke Yang,Wenguo Cui,Wei Jin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin light chain 3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody (AB233220)

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

Western blot - Anti-Myosin light chain 3 antibody (AB233220)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise